Silkworm hemolymph inhibits baculovirus-induced insect cell apoptosis.
Silkworm hemolymph inhibits baculovirus-induced insect cell apoptosis.
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DOI：
10.1006/bbrc.2000.2592
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发表时间：
2000-04
影响因子：
3.1
通讯作者：
W. Rhee;T. Park
中科院分区：
文献类型：
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作者：
W. Rhee;T. Park
研究了家蚕血淋巴对杆状病毒诱导的昆虫细胞凋亡的影响。在杆状病毒感染前或感染过程中向培养基中加入家蚕血淋巴可延长宿主细胞的寿命;然而，在感染后加入家蚕血淋巴的效果较差。这可以用家蚕血淋巴的较高转移率来解释，这是由于病毒内化步骤中的内吞作用引起的。家蚕血淋巴引起的延迟细胞死亡不是由抑制病毒附着和内化步骤引起的。利用DNA片段化和TUNEL法分析细胞凋亡，结果证实家蚕血淋巴抑制杆状病毒诱导的昆虫细胞凋亡。
The effect of silkworm hemolymph on baculovirus-induced insect cell apoptosis was investigated. The addition of silkworm hemolymph into the culture medium either before or during the baculovirus infection increased the host cell longevity; however, its addition after the infection was less effective. This can be explained by the higher transfer rate of silkworm hemolymph which is caused by endocytosis during the virus internalization step. The delayed cell death due to silkworm hemolymph was not caused by an inhibition of the virus attachment and internalization steps. The apoptosis was analyzed using DNA fragmentation and TUNEL assays, and the resulting data confirm that silkworm hemolymph inhibits baculovirus-induced insect cell apoptosis.