Effects of Testosterone on Production of Perivitelline Membrane Glycoprotein ZPC by Granulosa Cells of Japanese Quail (Coturnix japonica)1
Effects of Testosterone on Production of Perivitelline Membrane Glycoprotein ZPC by Granulosa Cells of Japanese Quail (Coturnix japonica)1
复制标题
睾酮对日本鹌鹑 (Coturnix japonica) 颗粒细胞产生卵周膜糖蛋白 ZPC 的影响1
作者：
Jianzhi Pan;T. Sasanami;Y. Kono;T. Matsuda;M. Mori
摘要鸡卵周膜是一种与哺乳动物卵母细胞透明膜同源的包被物，由至少两种糖蛋白组成。先前的研究表明，其中一个成分，一种与哺乳动物ZPC同源的糖蛋白，由鸡卵巢发育卵泡的颗粒细胞产生和分泌。在本研究中，我们评估了ZPC在日本鹌鹑（Coturnix japonica）颗粒细胞在体内和体外的表达和调节。使用抗鹌鹑ZPC抗血清对SDS溶解的颗粒层进行Western印迹分析表明，ZPC的量与卵泡发育平行增加。北方印迹分析表明，鹌鹑ZPC的mRNA表达的增加也与卵泡发育相关。为了研究ZPC产生的调节，将颗粒细胞培养在含有类固醇激素如孕酮、雌二醇-17 β或睾酮的培养基中。分别用Western和北方印迹分析测定ZPC蛋白和mRNA，我们发现，添加睾酮维持了颗粒细胞培养物中ZPC的含量，并且与对照相比，添加睾酮的培养物中ZPC mRNA的表达较高。这些结果表明，睾酮在基因转录水平上刺激ZPC蛋白的产生。
Abstract Avian perivitelline membrane, an investment homologous to the zona pellucida of mammalian oocytes, is composed of at least two glycoproteins. Previous studies have indicated that one of the components, a glycoprotein homologous to mammalian ZPC, is produced and secreted by the granulosa cells of developing follicles of the chicken ovary. In the present study, we evaluated the expression and regulation of ZPC in Japanese quail (Coturnix japonica) granulosa cells both in vivo and in vitro. Western blot analysis of the SDS-solubilized granulosa layer using anti-quail ZPC antiserum showed that the amount of ZPC increased in parallel with follicular development. Northern blot analysis of total RNA using cDNA of quail ZPC showed that the increase in mRNA expression was also correlated with follicular development. To investigate the regulation of ZPC production, the granulosa cells were cultured in a medium containing steroid hormones such as progesterone, estradiol-17β, or testosterone. By measuring ZPC protein and mRNA with Western and Northern blot analyses, respectively, we found that addition of testosterone maintained ZPC contents in the culture of the granulosa cells, and that ZPC mRNA expression was high in the culture with testosterone compared to the control. These results suggest that testosterone stimulates ZPC protein production at the gene transcription level.
DOI：
10.1016/s0021-9258(18)61070-1
发表时间：
1987-07
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
P. Matsudaira
通讯作者：
P. Matsudaira
兔卵泡中 55-kDa 透明带蛋白和信使 RNA 的发育表达。
影响因子：
2.7
作者：
Lee,VH;Dunbar,BS
通讯作者：
Dunbar,BS