Expression patterns and subcellular localization of porcine (Sus Scrofa) lectin, galactose-binding, soluble 1 gene.
Expression patterns and subcellular localization of porcine (Sus Scrofa) lectin, galactose-binding, soluble 1 gene.
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DOI：
10.1111/j.1745-7270.2008.00373.x
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发表时间：
2008
影响因子：
3.7
通讯作者：
H. Qiu;Shuhong Zhao;Mei Yu;B. Fan;Bang Liu
中科院分区：
文献类型：
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作者：
H. Qiu;Shuhong Zhao;Mei Yu;B. Fan;Bang Liu
半乳糖凝集素结合可溶性蛋白1（LGALS 1）基因编码半乳糖凝集素-1（galectin-1），半乳糖凝集素-1是一种在成肌细胞增殖和分化过程中起重要作用的非典型分泌蛋白。本研究从猪肌肉中克隆了猪LGALS 1基因并对其进行了鉴定。预测的蛋白质序列与其哺乳动物对应物具有高度的同一性。逆转录聚合酶链反应显示，猪LGALS 1在交配后33天和65天表达相对较高的水平，然后下降到90 dpc在胚胎骨骼肌发育，表明galectin-1是一个潜在的因素参与形成的肌纤维。LGALS-1在所有组织中广泛表达，瞬时转染表明galectin-1定位于细胞质和细胞核。基因组测序和分析预测启动子区在约1.279-1.529 kb，但双荧光素酶报告基因分析表明它几乎没有启动子活性。
Lectin, galactose-binding, soluble 1 (LGALS1) gene encodes galectin-1, an atypical secretory protein that plays an important role during myoblast proliferation and differentiation. In this study, the porcine LGALS1 gene was cloned and characterized from pig muscle. The predicted protein sequence shared a high identity with its mammalian counterparts. Reverse transcription-polymerase chain reaction revealed that porcine LGALS1 was expressed at 33 day post-coitus (dpc) and 65 dpc at a relatively high level, and then decreased to 90 dpc during fetal skeletal muscle development, suggesting that galectin-1 is a potent factor implicated in the formation of myofibers. LGALS1 was found widely expressed in all tissues and transient transfection indicated that galectin-1 locates both in cytoplasm and nucleus. Genomic sequences and analysis predicted a promoter region at approximately 1.279-1.529 kb, but dual-luciferase reporter assay indicated that it has little promoter activity.