MOTILITY AS A FACTOR IN THE COLONIZATION OF GNOTOBIOTIC PIGLETS BY HELICOBACTER-PYLORI
MOTILITY AS A FACTOR IN THE COLONIZATION OF GNOTOBIOTIC PIGLETS BY HELICOBACTER-PYLORI
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DOI：
10.1099/00222615-37-2-123
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发表时间：
1992-08-01
影响因子：
3
通讯作者：
KRAKOWKA, S
中科院分区：
文献类型：
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作者：
EATON, KA;MORGAN, DR;KRAKOWKA, S
幽门螺杆菌(26695株)出现非运动变异的频率为1.6(SD0.4)×10(-4)个变异/细胞/分裂周期，回复到运动形式的频率为10(-7)个变异/细胞/分裂周期。这两种形式在多达50次的细胞分裂中保持了90%的纯度，唯一的区别是有没有运动性和鞭毛。口服接种了可移动的幽门螺杆菌的10头诺生仔猪中，有9头恢复了细菌，但接种了非移动变种的8头小猪中，只有2头恢复了细菌。在感染的仔猪中，运动型存活21天，而非运动型仅存活6天。从接种了非运动性变种的仔猪中恢复的细菌是非运动性的。这些数据支持运动性是幽门螺杆菌定植因素的假设。
Non-motile variants of Helicobacter pylori (strain 26695) occurred with a frequency of 1.6 (SD 0.4) x 10(-4) variants/cell/division cycle, and reversion to the motile form occurred with a frequency of < 10(-7) variants/cell/division cycle. The two forms remained > 90 % pure for up to 50 cell divisions and differed only in the presence or absence of motility and flagella. Bacteria were recovered from nine of 10 gnotobiotic piglets inoculated orally with motile H. pylori, but from only two of eight inoculated with the non-motile variant. The motile form survived for 21 days in infected piglets, but the non-motile variant survived for only 6 days. Bacteria recovered from piglets inoculated with the non-motile variant were non-motile. These data support the hypothesis that motility is a colonisation factor for H. pylori.