Molecularly Imprinted Photo-electrochemical Sensor for Human Epididymis Protein 4 Based on Polymerized Ionic Liquid Hydrogel and Gold Nanoparticle/ZnCdHgSe Quantum Dots Composite Film
Molecularly Imprinted Photo-electrochemical Sensor for Human Epididymis Protein 4 Based on Polymerized Ionic Liquid Hydrogel and Gold Nanoparticle/ZnCdHgSe Quantum Dots Composite Film
复制标题
基于聚合离子液体水凝胶和金纳米颗粒/ZnCdHgSe量子点复合膜的人附睾蛋白4分子印迹光电化学传感器
DOI：
10.1021/acs.analchem.7b03486
复制
发表时间：
2017-11-21
影响因子：
7.4
通讯作者：
Li, Chunya
中科院分区：
文献类型：
--
作者：
Wang, Caiyun;Ye, Xiaoxue;Li, Chunya
合成了一种新型离子液体3-{[{4-[（（氨甲酰基）氨基）乙基甲基丙烯酸酯]丁基}（（氨甲酰基）氨基）乙基甲基丙烯酸酯]丙基}-1-乙烯基-1H-咪唑-3-溴化盐（CCPEimBr），并通过H-1 NMR、FTIR和HPLC-MS等技术对其进行了表征。以CCPEimBr为功能单体，在玻碳电极表面制备了分子印迹聚合离子液体水凝胶膜，用于人附睾蛋白4（HE 4）的传感。将金纳米粒子（AuNPs）和ZnCdHgSe量子点作为光电活性材料引入印迹膜中。测量光电流响应以研究印迹传感器对HE 4的传感性能。该印迹光电化学传感器具有良好的选择性、灵敏度、稳定性和准确性。对测定HE 4的孵育时间、pH值等实验条件进行了优化。光电流变化（Δ I）随HE 4浓度（c（HE 4））的增加而减小，在25 ~ 4.0 ng mL（-1）范围内与C-HE 4成线性关系。该印迹传感器对HE 4的检测限为15.4 pg/mL（-1）（S/N = 3）。将该印迹光电化学传感器用于人血清样品中HE 4的准确测定。
A novel ionic liquid, 3-{[{4-[((carbamoyl)-amino) ethyl methacrylate]butyl} ((carb amoyl) amino) ethyl methacrylate]propyl}-1-ethenyl-1H-imidazol-3-ium bromide (CCPEimBr) functionalized with vinyl, amino, and methacrylate groups, was synthesized and characterized with H-1 NMR, FTIR, and HPLC-MS techniques. CCPEimBr was adopted as the functional monomer to prepare a molecularly imprinted polymerized ionic liquid hydrogel film on a glassy carbon electrode surface for human epididymis protein 4 (HE4) sensing. Gold nanoparticles (AuNPs) and ZnCdHgSe QDs were incorporated into the imprinted film as photoelectric active materials. The photocurrent response was measured to investigate the sensing performance of the imprinted sensors toward HE4. The imprinted photo-electrochemical sensor shows excellent selectivity, sensitivity, stability, and accuracy for HE4 determination. Experimental conditions including incubation time and pH value for determining HE4 were optimized in this study. The photocurrent variation (Delta I) decreased with increasing HE4 concentration (c(HE4)), and it was linearly proportional to C-HE4 varied from 25 pg mL(-1) to 4.0 ng mL(-1). The detection limit of the imprinted sensor for determining HE4 was estimated to be 15.4 pg mL(-1) (S/N = 3). The imprinted photo-electrochemical sensor was used to determine HE4 in human serum samples accurately.