Determination of adenine nucleotides and inosine in human myocard by ion-pair reversed-phase high-performance liquid chromatography.
Determination of adenine nucleotides and inosine in human myocard by ion-pair reversed-phase high-performance liquid chromatography.
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离子对反相高效液相色谱法测定人心肌中的腺嘌呤核苷酸和肌苷。
DOI：
10.1016/s0021-9673(00)87253-2
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发表时间：
1982
影响因子：
4.1
通讯作者：
M. Farstad
中科院分区：
文献类型：
--
作者：
O. Ingebretsen;A. Bakken;L. Segadal;M. Farstad
本文建立了一种测定AMP、ADP和ATP的等度高效液相色谱系统。在室温下通过反相色谱法（Supelcosil LC-18）实现分离。标准溶剂为220 mM磷酸钾（pH 6.9）、1%（v/v）甲醇和0.3 mM四丁基硫酸氢铵。在这些实验条件下，观察到腺嘌呤核苷酸作为一个组相对于鸟苷、尿苷和胞苷的单磷酸、二磷酸和三磷酸的选择性保留。所采用的方法用于生物提取物中腺嘌呤核苷酸的分离，人类心肌在不到20分钟的时间内定量的腺嘌呤核苷酸的提取物的心肌。只有5-10毫克（湿重）的心肌被需要，以确定心肌样品的能量电荷。肌苷也很容易在该液相色谱系统定量。
An isocratic high-performance liquid chromatographic system for the quantitation of AMP, ADP and ATP is presented. The separations were achieved at room temperature by reversed-phase chromatography (Supelcosil LC-18). The standard solvent was 220 mMpotassium phosphate, pH 6.9, 1% (v/v) methanol and 0.3 mMtetrabutylammonium hydrogen sulphate. A selective retention of the adenine nucleotides as a group relative to the mono-, di- and triphosphates of guanosine, uridine and cytidine was observed under these experimental conditions. The adopted procedure was applied to the separation of adenine nucleotides in biological extracts,i.e.,human myocard. The adenine nucleotides in an extract of myocard were quantiated in less than 20 min. Only 5—10 mg (wet weight) of myocard were needed in order to determine the energy charge of a myocardial sample. Also inosine was easily quantitated in this liquid chromatographic system.