Functional characterization of a SAP protein expressed in dormant buds of peach
Functional characterization of a SAP protein expressed in dormant buds of peach
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发表时间：
2014
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通讯作者：
G. Ríos;A. Conejero;C. Leida;C. Petri;L. Burgos;M. Badenes
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作者：
G. Ríos;A. Conejero;C. Leida;C. Petri;L. Burgos;M. Badenes
在温带和北方气候中，许多多年生植物的分生组织在秋季和冬季的寒冷时期保持在芽内的循环静止状态，这确保了对寒冷和水分胁迫的影响的保护。光周期和温度控制生长停止和芽休眠的诱导，而休眠的解除需要对寒冷的定量感知。本研究采用两种转录组学方法对桃（Prunus persica [L.] Batsch）。在参与休眠、花粉发育和胁迫耐受性转录调控的不同基因中，我们鉴定了一个编码类似于胁迫相关蛋白（SAP）的蛋白的基因，该蛋白含有两个特定的锌指结构域，命名为A20和AN1（PpSAP）。SAP被描述为植物物种非生物胁迫反应的调节剂，成为提高植物胁迫耐受性的潜在候选物。我们研究了PpSAP在生殖芽和营养组织中的发育和胁迫依赖性表达。PpSAP在叶片和休眠芽中表达量较高，芽休眠解除后和芽萌发前表达量下调。PpSAP强烈的相互作用与泛素蛋白在酵母双杂交系统中，根据以前的工作支持SAP蛋白的E3泛素连接酶活性。PpSAP在转CaMV 35S启动子的控制下在转基因李植株中组成型表达。PpSAP的过表达导致叶片形状的改变和对叶片脱水的耐受性的增加，这赋予该基因在植物非生物胁迫耐受性的操纵方面的高度兴趣。
The meristems of many perennial plants in temperate and boreal climates remain in a cyclic quiescent state within buds during the cold period of autumn and winter, which ensures protection against the effects of cold and water stress. Photoperiod and temperature control growth cessation and bud dormancy induction, whereas dormancy release requires the quantitative perception of chilling. We performed two transcriptomic approaches for the identification of differently expressed transcripts in reproductive buds of peach (Prunus persica [L.] Batsch). Among different genes involved in transcriptional regulation of dormancy, pollen development and stress tolerance, we identified a gene coding for a protein similar to Stress Associated Proteins (SAP) containing two specific Zn-finger domains named A20 and AN1 (PpSAP). SAPs have been described as regulators of the abiotic stress response in plant species, emerging as potential candidates for improvement of stress tolerance in plants. We have studied the developmental and stress dependent expression of PpSAP in reproductive buds and vegetative tissues. PpSAP was highly expressed in leaves and dormant buds, being downregulated after the release of bud dormancy and before bud break. PpSAP strongly interacted with ubiquitin proteins in the yeast two-hybrid system, in accordance with previous works supporting an E3 ubiquitin ligase activity for SAP proteins. PpSAP was constitutively expressed in transgenic plum plants under the control of CaMV 35S promoter. PpSAP over-expression led to alterations in leaf shape and an increased tolerance to leaf desiccation, which conferred to this gene a high interest for the manipulation of abiotic stress tolerance in plants.