Time-dependent variation in the biodistribution of C60 in rats determined by liquid chromatography-tandem mass spectrometry
Time-dependent variation in the biodistribution of C60 in rats determined by liquid chromatography-tandem mass spectrometry
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DOI：
10.1016/j.toxlet.2011.07.010
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发表时间：
2011-10-10
影响因子：
3.5
通讯作者：
Nishimura, Tetsuji
中科院分区：
文献类型：
--
作者：
Kubota, Reiji;Tahara, Maiko;Nishimura, Tetsuji
采用LC-MS/MS研究了大鼠尾静脉给药后C-60的生物分布，并在大鼠脑、肾、肝、肺、脾等组织中检测到C-60。另一方面，在血液中没有发现C-60。C-60浓度在肺中最高，其次是脾、肝、肾和脑。这些结果表明，尾静脉注射的C-60可被肺毛细血管过滤并在肺内蓄积，然后分布到其他组织。此外，在血液中没有检测到C-60，这表明通过过滤从血液中清除C-60可能有效地发生在肺部。评价了C-60的生物分布随时间的变化。除脾组织外，所有组织中的C-60浓度均呈时间依赖性下降。此外，不同组织中C-60水平的下降趋势不同，这可能是由于积累的差异所致。这些结果表明，未经代谢酶修饰的C-60和/或C-60代谢物可被排入粪便和/或尿液。在进一步的研究中，应评估C-60的代谢和排泄途径，以了解C-60的毒代动力学。(C)2011爱思唯尔爱尔兰有限公司。保留所有权利。
We examined the biodistribution of C-60 in rats after tail vein administration using LC-MS/MS. C-60 was detected in various tissues, such as brain, kidneys, liver, lungs, and spleen of rats. On the other hand, no C-60 was found in blood. The highest C-60 concentration was observed in the lungs, followed by spleen, liver, kidneys, and brain. These results suggested that C-60 injected in the tail vein could be filtered by lung capillary vessels and accumulate in the lungs prior to being distributed to other tissues. Moreover, C-60 not being detected in the blood indicates that clearance of C-60 from the blood by filtration might effectively occur in the lungs. The time-dependent variation in the biodistribution of C-60 was evaluated. A time-dependent decrease in C-60 concentrations was observed in all tissues, except spleen. Moreover, a decreasing trend of C-60 levels differed among tissues, which could be due to differences in accumulation. These results suggest that unmodified C-60 and/or C-60 metabolites by metabolic enzymes could be excreted into feces and/or urine. In further studies, the metabolic and excretion pathways of C-60 should be evaluated to understand the toxicokinetics of C-60. (C) 2011 Elsevier Ireland Ltd. All rights reserved.