Effect of Paenibacillus alvei, strain K165, on the germination of Verticillium dahliae microsclerotia in planta
Effect of Paenibacillus alvei, strain K165, on the germination of Verticillium dahliae microsclerotia in planta
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DOI：
10.1016/j.biocontrol.2008.05.003
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发表时间：
2008-08-01
影响因子：
4.2
通讯作者：
Tjamos, Eleftherios C.
中科院分区：
文献类型：
--
作者：
Antonopoulos, Dimitrios F.;Tjamos, Sotirios E.;Tjamos, Eleftherios C.
黄萎病是由土传真菌黄萎病引起的一种危害广泛的草本植物和木本植物的病害。本文研究了生防潜力菌株--腺泡拟青霉K165菌株对大丽叶枯萎病菌(V.dahliae microscore，MSC)萌发的影响。K165菌株是从番茄根尖上分离到的一株菌株，温室和田间试验表明，该菌株对大丽叶枯萎病菌具有一定的防治活性。在本研究中，K165的应用使大丽叶病毒在茄子根尖和伸长区的MSC萌发率比对照降低了50%，而在距根尖10 cm和12 cm的无植物土壤中，MSC发芽率分别降低了26%和40%。然而，K165对每个萌发的MSC的菌丝数量和长度没有显著影响。在分根系统中，K165通过使病情严重程度和MSC萌发率分别降低27%和20%，从而诱导茄子对大丽叶病毒的系统抗性。此外，K165在茄子根际和土壤中的定殖密度分别为5和3log(10)cfu g(-1)、7dpi。这是首次报道了根际细菌对茄子根际MSC萌发的直接/间接影响，表明菌株K165降低了MSC的萌发。(C)2008 Elsevier Inc.保留所有权利。
Verticillium wilt is a devastating disease of a wide range of herbaceous and woody plant hosts, incited by the soilborne fungus Verticillium dahliae. In the present study, the effect of the potential biocontrol isolate Paenibacillus alvei, strain K165, on the germination of V. dahliae microsclerotia (msc) was investigated. Strain K165 was isolated from tomato root tips and its activity against V. dahliae has been shown in glasshouse and field experiments. In the present study, the application of K165 resulted in the reduction of msc germination of V. dahliae, in the root tips and the zone of elongation, of eggplants by 50% compared to the control treatment; whereas 10 and 12 cm away from root tips and in soil without plants the percentage of msc germination was reduced by 26% and 40%, respectively. However, K165 did not significantly affect the number and length of hyphae per germinated msc. In a split-root system, K165 triggered induced systemic resistance in eggplants against V. dahliae by reducing disease severity and msc germination by 27% and 20%, respectively. In addition, K165 colonised the rhizosphere of eggplants and soil in a population density of 5 and 3 log(10) cfu g(-1), 7 dpi, respectively. This is the first report of evaluating the direct/indirect effect of a rhizospheric bacterium on msc germination in the rhizosphere of eggplants, indicating that strain K165 reduces msc germination. (C) 2008 Elsevier Inc. All rights reserved.