Characterization of bacterial symbionts in Frankliniella occidentalis (Pergande), Western flower thrips
Characterization of bacterial symbionts in Frankliniella occidentalis (Pergande), Western flower thrips
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DOI：
10.1016/j.jip.2008.09.001
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发表时间：
2008-11-01
影响因子：
3.4
通讯作者：
Ullman, Diane
中科院分区：
文献类型：
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作者：
Chanbusarakum, Lisa;Ullman, Diane
许多昆虫与细菌有联系，尽管通常很难确定这种关系的复杂性。在一个这样的情况下，兼性细菌已被发现在一个主要的作物害虫和病毒载体，西方花蓟马（WFT），花蓟马（Pergande）（Thysanoptera：蓟马科）。在荷兰温室蓟马种群中研究了几种细菌分离物，分子数据表明这些细菌与大肠杆菌相似，尽管生化特性表明这些微生物实际上可能与欧文氏菌属中的植物病原菌最相似。我们专注于夏威夷群岛蓟马种群的细菌植物群，这些肠道细菌于1989年首次报道。我们还分析了德国人口和1965年加州人口保存在乙醇中。文化和文化独立的技术揭示了一致的微生物菌群，这是相似的荷兰分离株研究。来自多个种群和环境的蓟马微生物之间的相似性表明这些细菌和它们的宿主有着广泛的关联。根据16S rRNA基因和生化分析结果，推测蓟马中存在两个不同的微生物类群。系统发育分析还显示，支持一个蓟马细菌群与欧文氏菌属有共同的祖先，而第二组蓟马细菌与E。大肠杆菌，但没有支持。尽管由于16S的保守性，种间关系尚不确定，但有强烈的迹象表明，蓟马共生体属于两个不同的属，起源于环境微生物。(C)2008年爱思唯尔公司All rights reserved.
Many insects have associations with bacteria, although it is often difficult to determine the intricacies of the relationships. In one such case, facultative bacteria have been discovered in a major crop pest and virus vector, the Western flower thrips (WFT), Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae). Several bacterial isolates have been studied in Netherlands greenhouse thrips populations, with molecular data indicating that these bacteria were similar to Escherichia coli, although biochemical properties suggested these microbes might actually be most similar to plant pathogenic bacteria in the genus Erwinia. We focused on the bacterial flora of the Hawaiian Islands thrips population where these gut bacteria were first reported in 1989. We also analyzed a German population and a 1965 California population preserved in ethanol. Culture and culture-independent techniques revealed a consistent microflora that was similar to the Netherlands isolates studied. The similarity among thrips microbes from multiple populations and environments suggested these bacteria and their hosts share a widespread association. Molecular phylogeny based oil the 16S rRNA gene and biochemical analysis of thrips bacteria Suggested two distinctive groups of microbes are present in thrips. Phylogenetic analysis also revealed Support for one thrips bacterial group having a shared ancestry with Erwinia, whereas the second group of thrips bacteria fell Out with E. coli, but Without Support. Although species-specific relationships were indeterminable due to the conservative nature of 16S, there is strong indication that thrips symbionts belong to two different genera and originated from environmental microbes. (C) 2008 Elsevier Inc. All rights reserved.