Elevation of plasma ACTH concentration in rabbits made febrile by systemic injection of bacterial endotoxin
Elevation of plasma ACTH concentration in rabbits made febrile by systemic injection of bacterial endotoxin
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全身注射细菌内毒素致发热家兔血浆ACTH浓度升高
DOI：
10.1016/0006-8993(94)90228-3
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发表时间：
1994
期刊：
影响因子：
2.9
通讯作者：
J. M. Lipton
中科院分区：
文献类型：
--
作者：
Tatsuo Watanabe;W. G. Clark;G. Ceriani;J. M. Lipton
本研究观察了全身注射内毒素致家兔发热时促肾上腺皮质激素（ACTH）的变化。静脉注射（i. v.）注射LPS（0.1 μg/kg和1.0 μg/kg）以剂量相关的方式增加直肠温度（双相热）和血浆ACTH浓度（ACTH反应）。用吲哚美辛（20 mg/kg，皮下注射）预处理可抑制这些反应。脑室内（i. c. v.）吲哚美辛（400 μg）对ACTH对LPS的反应无影响，但能显著抑制发热反应。前列腺素E2（2 μg）或F2α（2 μ g）i. c. v.给药后，ACTH血浆浓度小幅升高，并出现明显发热。静脉注射促肾上腺皮质激素释放因子（CRF）拮抗剂[α-螺旋CRF（9-41）（200 μg/kg）]可部分抑制LPS诱导的血浆ACTH升高。这些结果表明，在血脑屏障外合成的胡萝卜素在促肾上腺皮质激素反应中起着重要作用，并且诱导促肾上腺皮质激素反应的机制与发热反应的机制并不完全相同，尽管胡萝卜素参与了这两种反应。
This study was carried out to investigate the adrenocorticotrophic hormone (ACTH) response in rabbits made febrile by systemic injection of lipopolysaccharide (LPS,Salmonella typhosaendotoxin). Intravenous (i.v.) injection of LPS (0.1 μg/kg and 1.0 μg/kg) increased rectal temperature (biphasic fever) and the plasma concentration of ACTH (ACTH response) in a dose-related manner. These responses were suppressed by pretreatment with indomethacin (20 mg/kg, subcutaneously). Intracerebroventricular (i.c.v.) administration of indomethacin (400 μg) had no effect on the ACTH response to LPS, although it significantly suppressed febrile response. Small increases in plasma concentration of ACTH and significant fevers followed i.c.v. administration of prostaglandin E2(2 μg) or F2α(2 μg). I.v. administration of corticotropin releasing factor (CRF) antagonist [α-helical CRF (9–41) (200 μg/kg)] partly suppressed the ACTH increased induced in plasma by i.v. LPS. These results suggest that prostagandins synthesized outside the blood-brain barrier play an important role in the ACTH response and that the mechanism for induction of the ACTH response is not exactly the same as that for the febrile response, although prostaglandins are involved in both responses.