Quantitative Changes in the Transcription of Phytohormone-Related Genes: Some Transcription Factors Are Major Causes of the Wheat Mutant dmc Not Tillering.
Quantitative Changes in the Transcription of Phytohormone-Related Genes: Some Transcription Factors Are Major Causes of the Wheat Mutant dmc Not Tillering.
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DOI：
10.3390/ijms19051324
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发表时间：
2018-04-29
影响因子：
5.6
通讯作者：
Niu J
中科院分区：
文献类型：
--
作者：
He R;Ni Y;Li J;Jiao Z;Zhu X;Jiang Y;Li Q;Niu J
小麦分蘖数是决定小麦产量的重要农艺性状。从栽培品种国麦301（野生型，WT）中获得了一个矮秆单茎小麦突变体（dmc）。在此，我们探讨了dmc突变体抑制分蘖发育的分子基础。通过混合RNA测序（RNA-Seq）在转录水平上比较分析具有三个生物学重复的突变体dmc（T1、T2和T3）和WT（T4、T5和T6）的两个混合样品。总共产生了68.8 Gb数据和4.63亿个读段，其中80%被定位到中国春小麦参考基因组。在突变体dmc和WT之间共鉴定出4904个差异表达基因（DEG）。基于GO（Gene Ontology）和KEGG（京都基因和基因组百科全书）分类，对DEG及其相关的主要生物学功能进行了表征。这些结果通过经由实时qRT-PCR定量分析12个选择的DEG的表达谱来证实。与植物激素合成相关的生长素、玉米素、细胞分裂素和TALE、WOX等转录因子家族的基因表达下调可能是导致突变体dmc的主要原因，而不是分蘖。本研究为以后的分蘖发育研究奠定了基础。
Tiller number is an important agronomic trait for grain yield of wheat (Triticum aestivum L.). A dwarf-monoculm wheat mutant (dmc) was obtained from cultivar Guomai 301 (wild type, WT). Here, we explored the molecular basis for the restrained tiller development of the mutant dmc. Two bulked samples of the mutant dmc (T1, T2 and T3) and WT (T4, T5 and T6) with three biological replicates were comparatively analyzed at the transcriptional level by bulked RNA sequencing (RNA-Seq). In total, 68.8 Gb data and 463 million reads were generated, 80% of which were mapped to the wheat reference genome of Chinese Spring. A total of 4904 differentially expressed genes (DEGs) were identified between the mutant dmc and WT. DEGs and their related major biological functions were characterized based on GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) categories. These results were confirmed by quantitatively analyzing the expression profiles of twelve selected DEGs via real-time qRT-PCR. The down-regulated gene expressions related to phytohormone syntheses of auxin, zeatin, cytokinin and some transcription factor (TF) families of TALE, and WOX might be the major causes of the mutant dmc, not tillering. Our work provides a foundation for subsequent tiller development research in the future.
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