Drought tolerance induction in transgenic tobacco through RNA interference of BrDST71, a drought-responsive gene from Chinese cabbage
Drought tolerance induction in transgenic tobacco through RNA interference of BrDST71, a drought-responsive gene from Chinese cabbage
复制标题
DOI：
10.1007/s13580-018-0070-7
复制
发表时间：
2018-08
期刊：
影响因子：
--
通讯作者：
J. Park;Jae-Gyeong Yu;Gi-Ho Lee;Young-Doo Park
中科院分区：
文献类型：
--
作者：
J. Park;Jae-Gyeong Yu;Gi-Ho Lee;Young-Doo Park
由于干旱是导致严重农业问题的主要环境因素，了解植物对干旱胁迫的反应机制和遗传基础是至关重要的。利用Brassica rapa135K基因芯片对BrDST71基因进行了鉴定，结果表明，BrDST71在抗旱大白菜中的表达比野生型降低了8倍，编码362个氨基酸的蛋白，含有一个分泌型过氧化物酶结构域。构建了BrDST71基因的高效表达载体和RNAi载体，并通过农杆菌介导法转化烟草。对BrDST71在干旱条件下的表达水平和表型进行了分析。与野生型和高表达转基因品系相比，BrDST71表达抑制的转基因品系表现出更强的抗旱性。这表明，抑制BrDST71的表达与干旱条件下更好的生长有关。基于这些结果，我们认为下调BrDST71基因可以提高植物的抗旱性。
Because drought is a major environmental factor that causes serious agricultural problems, understanding the mechanisms and genetic bases underlying plant responses to drought stress is essential. Using theBrassica rapa135 K microarray,BrDST71gene was identified.BrDST71expression in drought-tolerant Chinese cabbage showed an eight-fold decrease than in wild-type, and encodes a 362 amino acid protein containing a secretory peroxidase domain. Overexpression and RNAivectors ofBrDST71were constructed and each vector was transformed intoNicotiana tabacumby theAgrobacterium-mediated transformation method. The expression level ofBrDST71and the phenotype were analyzed under drought condition. Transgenic lines with suppressed expression ofBrDST71showed more tolerance to drought stress compared to wild-type and overexpression transgenic lines. It showed that suppressingBrDST71expression is correlated to better growth under drought conditions. Based on these results, we suggest that down-regulation ofBrDST71improves drought tolerance.