Oral administration of Lactobacillus brevis 23017 combined with ellagic acid attenuates intestinal inflammatory injury caused by Eimeria infection by activating the Nrf2/HO-1 antioxidant pathway.
Oral administration of Lactobacillus brevis 23017 combined with ellagic acid attenuates intestinal inflammatory injury caused by Eimeria infection by activating the Nrf2/HO-1 antioxidant pathway.
复制标题
DOI：
10.1186/s13567-022-01042-z
复制
发表时间：
2022-03-18
影响因子：
4.4
通讯作者：
Ma D
中科院分区：
文献类型：
--
作者：
Yang X;Pan X;Jia Z;Bai B;Zhi W;Chen H;Ma C;Ma D
本研究的目的是探讨单独口服短乳杆菌 23017 (LB) 以及与鞣花酸组合是否可以通过激活 Nrf2/HO-1 通路来抑制 ChTLR15/ChNLRP3/ChIL-1β，从而减轻肠道炎症损伤。进行了两项动物实验。在实验1中，将鸡分为7组：PBS、低、中、高剂量的活和热灭活的LB，分别命名为L/LB(+)、M/LB(+)和H/LB(+)，以及L/LB(−)、M/LB(−)和H/LB(−)。实验2将鸡分为5组：PBS、攻击对照、低、中、高剂量鞣花酸联合LB(+)，分别命名为L/EA + L/LB(+)、M/EA + M/LB(+)和H/EA + H/LB(+)。每天用含或不含鞣花酸的LB给鸡灌胃一次。然后，对盲肠组织中发现的 Nrf2/HO-1 通路成分的 mRNA 和蛋白质水平进行定量。感染柔嫩艾美球虫后第 7 天，再次定量盲肠中 ChTLR15/NLRP3/IL-1β 通路成分的水平，并评估抗球虫作用。结果显示，LB(+)组鸡Nrf2/HO-1通路基因水平高于LB(-)组(p<0.001)； H/LB(+)组高于M/LB(+)和L/LB(+)组(p < 0.001);与其他组相比，H/EA + H/LB(+)组的表达水平最高(p < 0.001)。攻击后，H/LB(+)组鸡的炎症损伤较M/LB(+)和L/LB(+)组少(p < 0.05)，H/EA + H/LB(+)组鸡的抗炎作用强于其他组(p < 0.05)。因此，这些针对感染的保护作用与上述结果一致。总体而言，在口服高剂量活短乳杆菌 23017 和鞣花酸的鸡中观察到显着的抗炎作用，这是通过调节 ChTLR15/NLRP3/IL-1β 途径而发生的。
The aim of this study was to investigate whether oral administration of Lactobacillus brevis 23017 (LB) alone and in combination with ellagic acid inhibits ChTLR15/ChNLRP3/ChIL-1β by activating the Nrf2/HO-1 pathway to attenuate intestinal inflammatory injury. Two animal experiments were performed. In Experiment 1, chickens were allocated into 7 groups: PBS, and low, medium and high dosages of live and heat-killed LB, named L/LB(+), M/LB(+) and H/LB(+), and L/LB(−), M/LB(−) and H/LB(−), respectively. In Experiment 2, chickens were divided into 5 groups: PBS, challenge control, and low, medium and high dosages of ellagic acid combined with LB(+), named L/EA + L/LB(+), M/EA + M/LB(+) and H/EA + H/LB(+), respectively. Chickens were gavaged with LB with or without ellagic acid once a day. Then, the mRNA and protein levels of the components of the Nrf2/HO-1 pathway found in the caecal tissues were quantified. On Day 7 post-infection with E. tenella, the levels of the components of the ChTLR15/NLRP3/IL-1β pathway in the caeca were again quantified, and the anticoccidial effects were assessed. The results showed that the levels of the genes in the Nrf2/HO-1 pathway in the chickens in the LB(+) groups were higher than those in the LB(−) groups (p < 0.001); those in the H/LB(+) group were higher than those in the M/LB(+) and L/LB(+) groups (p < 0.001); and those in the H/EA + H/LB(+) group showed the highest expression levels compared with the other groups (p < 0.001). After challenge, the chickens in the H/LB(+) group displayed less inflammatory injury than those in the M/LB(+) and L/LB(+) groups (p < 0.05), and the chickens in the H/EA + H/LB(+) group showed stronger anti-inflammatory effects than the other groups (p < 0.05). Thus, these protective effects against infection were consistent with the above results. Overall, significant anti-inflammatory effects were observed in chickens orally gavaged with high dosages of live L. brevis 23017 and ellagic acid, which occurred by regulation of the ChTLR15/NLRP3/IL-1β pathway.
登录
查看更多内容
影响因子：
24.1
作者：
Mulas F;Wang X;Song S;Nishanth G;Yi W;Brunn A;Larsen PK;Isermann B;Kalinke U;Barragan A;Naumann M;Deckert M;Schlüter D
通讯作者：
Schlüter D
DOI：
10.3390/ani10091484
发表时间：
2020-08-24
期刊：
Animals : an open access journal from MDPI
影响因子：
--
作者：
Song X;Li Y;Chen S;Jia R;Huang Y;Zou Y;Li L;Zhao X;Yin Z
通讯作者：
Yin Z
影响因子：
5.9
作者：
Hilliard A;Mendonca P;Russell TD;Soliman KFA
通讯作者：
Soliman KFA
影响因子：
5.6
作者：
Rahman MS;Alam MB;Kim YK;Madina MH;Fliss I;Lee SH;Yoo JC
通讯作者：
Yoo JC
DOI：
10.1073/pnas.1014156108
发表时间：
2011-01-25
影响因子：
11.1
作者：
Fujita, Ken-ichi;Maeda, Daisuke;Srinivasula, Srinivasa M.
通讯作者：
Srinivasula, Srinivasa M.