Expression detection of DMRTs and two sox9 genes in Takifugu rubripes (Tetraodontidae, Vertebrata).
Expression detection of DMRTs and two sox9 genes in Takifugu rubripes (Tetraodontidae, Vertebrata).
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DOI：
10.1007/s11802-007-0182-7
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发表时间：
2007-04-30
影响因子：
1.6
通讯作者：
Gu Qianqun
中科院分区：
文献类型：
--
作者：
Shen Xueyan;Cui Jianzhou;Gu Qianqun
性别决定和性别分化是鱼类重要的生理现象，但红鳍东方鲀性别决定的机制尚不清楚。在本研究中，我们对T.红足组织用逆转录（RT）-PCR检测。结果表明，DMRT 1在精巢和卵巢中的表达量较低，在肌肉、血液和尾鳍中没有表达。然而，在上述组织中未发现DMRT 2和DAMT 3的表达。sox 9a在肌肉、鳍、卵巢和精巢中有表达，而在血液中无表达，而sox 9 b仅在卵巢中有表达。DMRTs、sox 9a和sox 9 b在T.红足生殖腺表明，这些基因可能不是性别特异性的。
Sex determination and sex differentiation are important phenomena in fish, but the mechanisms of sex determination in Takifugu rubripes are poorly understood. In our study, the expression patterns of genes for DMRTs (DMRT1, DMRT2 and DMRT3), sox9a and sox9b in T. rubripes tissues were verified with the Reverse Transcription (RT)-PCR detection. It is showed that DMRT1 expressions in testis and ovaries were much lower, and no expressions were found in muscle, blood and tailfin. However, expressions for DMRT2 and DAMT3 were not found in the tissues stated above. Transcripts of sox9a were detected in muscle, fin, ovary and testis, but not in blood, whereas sox9b expression was only detected in ovary. The expression patterns of DMRTs, sox9a and sox9b in T. rubripes gonads suggest that these genes may not be sex-specific.