Poly (fumaroyl bioxirane) maleate: A potential functional scaffold for bone regeneration
Poly (fumaroyl bioxirane) maleate: A potential functional scaffold for bone regeneration
复制标题
聚（富马酰二环氧乙烷）马来酸酯：骨再生的潜在功能支架
DOI：
10.1016/j.msec.2017.02.164
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发表时间：
2017-07-01
影响因子：
7.9
通讯作者：
Fan, Xianqun
中科院分区：
文献类型：
--
作者：
Ding, Yi;Su, Yun;Fan, Xianqun
合适的支架材料结合间充质干细胞（MSCs）是修复骨缺损的一种很有前途的策略。在先前的研究中，聚马来酸双环氧丙酯（PFM），一种新开发的具有许多功能基团的功能聚合物，表现出良好的生物相容性，并在体外增强成骨细胞的碱性磷酸酶（ALP）活性。在这里，提供进一步和全面的了解PFM在骨组织工程中的应用，我们研究了PFM与大鼠脂肪间充质干细胞（rADSCs）培养的骨诱导潜力。结果表明，PFM导致更大的增殖rADSCs和PFM基板具有更强的骨诱导性比PLGA和对照，如在体外成骨相关基因，蛋白质和钙矿化的显着上调所示。接下来，将PFM与rADSCs组合以修复大鼠中临界尺寸的颅骨缺损。与PLGA支架相比，PFM支架明显促进新骨形成，对大鼠颅骨缺损具有良好的修复效果。结论：PPM具有较强的骨诱导活性，可显著促进骨再生，有望成为传统骨再生支架材料的一种有效优化方法。(C)2017爱思唯尔B.V.保留所有权利。
Proper scaffolds combined with mesenchymal stem cells (MSCs) represent a promising strategy for repairing bone defects. In a previous study, poly (fumaroyl bioxirane) maleate (PFM), a newly developed functional polymer with numerous functional groups, exhibited excellent biocompatibility and enhanced the alkaline phosphatase (ALP) activity of osteoblasts in vitro. Here, to provide further and comprehensive insight into the application of PFM in bone tissue engineering, we investigated the osteoinductive potential of PFM cultured with rat adipose derived mesenchymal stem cells (rADSCs). The results showed that PFM resulted in greater proliferation of rADSCs and that the PFM substrate had stronger osteoinductivity than PLGA and the control, as indicated by the significant upregulation of osteogenesis-related genes, proteins and calcium mineralization in vitro. Next, PFM was combined with rADSCs to repair a critical-sized calvarial defect in rats. Compared to the PLGA scaffold, the PFM scaffold significantly promoted new bone formation and exhibited excellent effects in repairing rat calvarial defects. In conclusion, PPM possesses strong osteoinductivity, which could markedly enhance bone regeneration, suggesting that PFM could serve as a promising and effective optimization method for traditional scaffolds in bone regeneration. (C) 2017 Elsevier B.V. All rights reserved.