Whole-mount X-Gal staining of mouse tissues.
Whole-mount X-Gal staining of mouse tissues.
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DOI：
10.1101/pdb.prot073452
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发表时间：
2014-04-01
影响因子：
--
通讯作者：
Haigis KM
中科院分区：
文献类型：
--
作者：
Gierut JJ;Jacks TE;Haigis KM
尽管改进的小鼠模型的发展，包括条件缺失，标志着小鼠遗传学上一个令人兴奋的时代，但表征和验证这些模型是重要的。Cre报告菌株允许研究人员评估重组酶在单个Cre小鼠品系中的表达谱和功能。这些菌株被设计成在去除有花丝的停止盒后表达报告基因(通常是LacZ)，从而标记可以被给定的Cre品系作为靶标的细胞系。该方法为Cre小鼠品系β-半乳糖苷酶活性的组织化学检测提供了一种详细的方法。
Although the development of improved mouse models, including conditional deletions, marks an exciting time in mouse genetics, it is important to characterize and validate these models. Cre reporter strains allow researchers to assess the recombinase expression profile and function in individual Cre mouse lines. These strains are engineered to express a reporter gene (usually LacZ) following the removal of a floxed STOP cassette, thus marking cell lineages that can be targeted with a given Cre line. This protocol provides a detailed method for the histochemical detection of β-galactosidase activity in Cre mouse strains.