LcBiP, a Endoplasmic Reticulum Chaperone Binding Protein Gene from Lycium chinense, Confers Cadmium Tolerance in Transgenic Tobacco
LcBiP, a Endoplasmic Reticulum Chaperone Binding Protein Gene from Lycium chinense, Confers Cadmium Tolerance in Transgenic Tobacco
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LcBiP，一种来自枸杞的内质网伴侣结合蛋白基因，赋予转基因烟草镉耐受性
DOI：
10.1002/btpr.2046
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发表时间：
2015-03-01
影响因子：
2.9
通讯作者：
Li, Xiaozhou
中科院分区：
文献类型：
--
作者：
Guan, Chunfeng;Jin, Chao;Li, Xiaozhou
镉（Cd）的积累对植物有很大的毒性。Cd的存在会导致活性氧（ROS）的过量产生，进而导致植物生长受到抑制。内质网伴侣结合蛋白（BiP）是一种重要的功能蛋白，已被证明是内质网环境变化的传感器。植物中BiP的过表达可以通过抑制ROS积累来提高抗旱性。鉴于上述关系，植物Cd胁迫耐受性、ROS积累与BiP转录物表达之间可能存在联系。本研究从羊草中分离并鉴定了一个BiP基因LcBiP。烟草中LcBiP的过表达使其具有Cd耐受性。在Cd胁迫条件下，转基因烟草的叶绿素保持能力较强，活性氧积累较少，根长较长，谷胱甘肽（GSH）含量较高，抗氧化酶活性较低。这些数据表明，lbip在镉胁迫耐受中起正调节作用。据推测，转基因烟草耐Cd能力的提高可能与活性氧清除能力的增强有关。GSH含量的增加可能与转基因植株清除ROS的能力有关。然而，BiP介导的镉胁迫耐受性增加的潜在机制需要进一步阐明。©2015美国化学工程师协会生物技术。掠夺。中文信息学报，31:358-368,2015
Cadmium (Cd) accumulation is very toxic to plants. The presence of Cd may lead to excessive production of reactive oxygen species (ROS), and then cause inhibition of plant growth. The endoplasmic reticulum chaperone binding protein (BiP) is an important functional protein, which has been shown to function as a sensor of alterations in the ER environment. BiP overexpression in plants was shown to increase drought tolerance through inhibition of ROS accumulation. Due to the above relationships, it is likely that there may be a link between Cd stress tolerance, ROS accumulation and the BiP transcript expression in plants. In this study, a BiP gene, LcBiP, from L. chinense was isolated and characterized. Overexpression of LcBiP in tobacco conferred Cd tolerance. Under Cd stress conditions, the transgenic tobacco lines exhibited better chlorophyll retention, less accumulation of ROS, longer root length, more glutathione (GSH) content, and less antioxidant enzyme activity than the wild type. These data demonstrated that LcBiP act as a positive regulator in Cd stress tolerance. It is hypothesized that the improved Cd tolerance of the transgenic tobacco plants may be due to the enhanced ROS scavenging capacity. The enhancement of GSH content might contribute to this ROS scavenging capacity in the transgenic plants. However, the underlying mechanism for BiP‐mediated increase in Cd stress tolerance need to be further clarified. © 2015 American Institute of Chemical Engineers Biotechnol. Prog., 31:358–368, 2015