Comparison of microbial communities during the anaerobic digestion of Gracilaria under mesophilic and thermophilic conditions
Comparison of microbial communities during the anaerobic digestion of Gracilaria under mesophilic and thermophilic conditions
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DOI：
10.1007/s11274-016-2112-6
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发表时间：
2016-10-01
影响因子：
4.1
通讯作者：
Lee, Jung Hyun
中科院分区：
文献类型：
--
作者：
Azizi, Aqil;Kim, Wonduck;Lee, Jung Hyun
通过分析其性能和微生物群落变化，对分别利用江蓠和海洋沉积物作为底物和接种物的中温和高温厌氧消化器（分别为MD和TD）进行比较。在连续三次转移期间，MD 和 TD 的平均累积甲烷产量分别为 222.6 +/- 17.3 mL CH4/g 挥发性固体 (VS) 和 246.1 +/- 11 mL CH4/g VS。与 MD 相比，TD 中较高的水解速率和酸生成导致挥发性脂肪酸（乙酸盐、丙酸盐和丁酸盐）的积累增加数倍，随后 pH 值下降幅度更大，恢复时间更长。然而，两个沼气池的运行稳定性仍然相当。焦磷酸测序分析表明，MD 比 TD 具有更复杂的微生物多样性指数和微生物群落变化。有趣的是，第七个产甲烷菌目 Methanomassiliicoccales 是 MD 中的主要古菌目，其他细菌目包括梭菌目、拟杆菌目和协同目。与此同时，嗜热杆菌和甲烷杆菌分别在 TD 的细菌和古菌群落中占主导地位。尽管甲烷产量相当，但 MD 和 TD 显示出不同的 pH、VFA 和微生物群落特征。
Mesophilic and thermophilic anaerobic digesters (MD and TD, respectively) utilizing Gracilaria and marine sediment as the substrate and inoculum, respectively, were compared by analyzing their performances and microbial community changes. During three successive transfers, the average cumulative methane yields in the MD and TD were 222.6 +/- 17.3 mL CH4/g volatile solids (VS) and 246.1 +/- 11 mL CH4/g VS, respectively. The higher hydrolysis rate and acidogenesis in the TD resulted in a several fold greater accumulation of volatile fatty acids (acetate, propionate, and butyrate) followed by a larger pH drop with a prolonged recovery than in the MD. However, the operational stability between both digesters remained comparable. Pyrosequencing analyses revealed that the MD had more complex microbial diversity indices and microbial community changes than the TD. Interestingly, Methanomassiliicoccales, the seventh methanogen order was the predominant archaeal order in the MD along with bacterial orders of Clostridiales, Bacteriodales, and Synergistales. Meanwhile, Coprothermobacter and Methanobacteriales dominated the bacterial and archaeal community in the TD, respectively. Although the methane yield is comparable, both MD and TD show a different profile of pH, VFA and the microbial communities.