Biofilm-forming a ability of Candida albicans is unlikely to contribute to high levels of oral yeast carriage in cases of human immunodeficiency virus infection
Biofilm-forming a ability of Candida albicans is unlikely to contribute to high levels of oral yeast carriage in cases of human immunodeficiency virus infection
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DOI：
10.1128/jcm.41.7.2961-2967.2003
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发表时间：
2003-07-01
影响因子：
9.4
通讯作者：
Samaranayake, LP
中科院分区：
文献类型：
--
作者：
Jin, Y;Yip, HK;Samaranayake, LP
在人类免疫缺陷病毒（HIV）感染的情况下，念珠菌携带和口腔念珠菌病的患病率增加是常见的，其原因可能包括定殖酵母在粘膜表面产生生物膜的能力增强。因此，本研究的目的是检查的差异，如果有的话，在生物膜形成能力的26个白色念珠菌酵母分离株的HIV感染者和20个分离株的HIV-免费的个人，因为这个属性的酵母分离株的HIV疾病患者之前没有检查。采用2，3-双（2-甲氧基-4-硝基-5-磺基苯基）-5-[（苯氨基）羰基]-2H-氢氧化四唑（XTT）还原法和结晶紫法定量测定了微量滴定板威尔斯孔中生物膜的形成。虽然两种方法均可定量评价念珠菌生物膜的形成，但XTT还原法的重现性优于结晶紫法（P < 0.05），因此前者更可靠。C.在多孔培养系统中体外孵育66小时期间，从HIV感染患者分离的白色念珠菌和从无HIV个体分离的白色念珠菌。在HIV感染组的8个宿主因素中，有3个与念珠菌生物膜的形成有关。因此，从老年个体和具有较高CD 4细胞计数的个体中分离的酵母表现出生物膜形成减少，而从接受齐多夫定的个体中分离的酵母的发现显示出相反的结果（对于所有比较，P < 0.05）。我们的数据表明，属性以外的生物膜形成可能有助于增加口腔酵母菌携带率在艾滋病毒感染的情况下。
An increased prevalence of candidal carriage and oral candidiasis is common in cases of human immunodeficiency virus (HIV) infection, and the reasons for this may include the enhanced ability of colonizing yeasts to produce biofilms on mucosal surfaces. The aim of the present study was therefore to examine the differences, if any, in the biofilm-forming abilities of 26 Candida albicans yeast isolates from HIV-infected individuals and 20 isolates from HIV-free individuals, as this attribute of yeast isolates from patients with HIV disease has not been examined before. Biofilm formation in microtiter plate wells was quantitatively determined by both the 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide (XTT) reduction method and the crystal violet method. Although candidal biofilm formation could be quantitatively evaluated by either technique, the better reproducibility (P < 0.05) of the XTT reduction assay compared with that of the crystal violet method led us to conclude that the former is more reliable. There were no significant quantitative differences in biofilm formation between C. albicans isolates from HIV-infected patients and isolates from HIV-free individuals during in vitro incubation in a multiwell culture system over a period of 66 h. Three of eight host factors in the HIV-infected group were found to be associated with candidal biofilm formation. Thus, yeasts isolated from older individuals and those with higher CD4-cell counts exhibited decreased biofilm formation, while the findings for yeasts from individuals receiving zidovudine showed the reverse (P < 0.05 for all comparison). Our data indicate that attributes other than biofilm formation may contribute to the increased oral yeast carriage rates in cases of HIV infection.