Cell-Cell adhesion molecule: Identification of a glycoprotein relevant to the Ca2+-independent aggregation of chinese hamster fibroblasts
Cell-Cell adhesion molecule: Identification of a glycoprotein relevant to the Ca2+-independent aggregation of chinese hamster fibroblasts
复制标题
细胞-细胞粘附分子：与中国仓鼠成纤维细胞的 Ca2 依赖性聚集相关的糖蛋白的鉴定
DOI：
10.1016/0092-8674(80)90622-4
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发表时间：
1980
期刊：
影响因子：
64.5
通讯作者：
M. Takeichi
中科院分区：
文献类型：
--
作者：
H. Urushihara;M. Takeichi
对构成中国仓鼠 V79 细胞的 Ca*+ 独立细胞粘附位点 (CIDS) 的分子进行了研究。先前的研究表明，针对V79细胞表面产生的抗体的Fab片段（Fab）抑制V79细胞的Ca*+非依赖性聚集，并且这种抑制作用可以通过添加用0.01%胰蛋白酶处理的V79细胞表面释放的一些蛋白质物质来中和。在本研究中，我们发现针对这种物质（抗 TRF）产生的抗体对具有活性 CIDS 的细胞比对没有活性 CIDS 的细胞表现出更强的补体依赖性细胞毒性。该抗体的 Fab 强烈抑制 V79 细胞的 Ca*+ 独立聚集，特别是在透明质酸酶存在的情况下。用放射性碘、单糖或蛋氨酸标记的细胞裂解物中的抗 TRF 进行免疫沉淀的材料的电泳表明，分子量为 125 的糖蛋白 DDD 是具有活性 CIDS 的细胞中与该抗体反应的唯一成分。抗TRF的间接免疫荧光显示该抗原分布在V79细胞的整个表面，并且仅存在于中国仓鼠肝细胞原代培养物的成纤维细胞中。这些结果表明，分子量为125的糖蛋白DDO在中国仓鼠成纤维细胞的Ca*+非依赖性粘附中具有特定作用。
Molecules making up the Ca*+-independent cell-cell adhesion sites (CIDS) of Chinese hamster V79 cells have been investigated. Previous studies showed that Fab fragments(Fab) of the antibody raised against the surface of V79 cells inhibit the Ca*+-independent aggregation of V79 cells, and that this inhibitory effect is neutralized by the addition of some proteinous substance released from the surface of V79 cells treated with 0.01% trypsin. In the present study, we found that the antibody raised against this substance (anti-TRF) displays the complement-dependent cytotoxicity more strongly to the cells with active CIDS than to those without it. The Fab of this antibody inhibited the Ca*+-independent aggregation of V79 cells strongly, particularly in the presence of a hyaluronidase. Electrophoresis of the materials immunoprecipitated with anti-TRF from lysates of cells labeled with radioactive iodine, monosaccharides or methionine showed that a glycoprotein with a molecular weight of 125, DDD is the only component to react with this antibody in cells with the active CIDS. Indirect immunofluorescence of anti-TRF showed that the antigens are distributed over the entire surface of V79 cells, and are present only in fibroblastic cells in the primary culture of Chinese hamster hepatic cells. These results suggest that the glycoprotein with a molecular weight of 125, DDO has a specific role in the Ca*+-independent adhesion of Chinese hamster fibroblastic cells.