In vitro synergy of ciprofloxacin and three other antibiotics against Bacteroides fragilis.
In vitro synergy of ciprofloxacin and three other antibiotics against Bacteroides fragilis.
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环丙沙星和其他三种抗生素对抗脆弱拟杆菌的体外协同作用。
DOI：
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发表时间：
1987
期刊：
影响因子：
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通讯作者：
H. Thadepalli
中科院分区：
文献类型：
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作者：
S. Esposito;A. Gupta;H. Thadepalli
评价了环丙沙星与美洛西林、头孢西丁、克林霉素联合应用对30株脆弱类杆菌的体外协同作用。所有细菌均为临床分离株，经气相色谱和碳水化合物发酵鉴定。通过微型化稀释肉汤法测定药物的MIC。对药物的抗性或敏感性不是选择菌株的标准。通过棋盘法使用脑底浸液肉汤和10（6）CFU/ml的细菌接种物测试抗菌组合。将微量滴定板在厌氧罐（BBL）中在37 ° C下孵育48小时。用预还原的碎肉碳水化合物肉汤保存和培养菌株。抗菌剂组合的效果是根据分数抑菌浓度（FIC）指数定义的。我们认为：协同效应FIC ≤ 0.5;相加效应FIC = 0.5-0.75;无关效应FIC = 0.76-2.0;拮抗效应FIC ≥ 2。环丙沙星+克林霉素对26株中的10株（38%）有协同作用;环丙沙星+美洛西林对30株中的9株（30%）有协同作用;环丙沙星+头孢西丁对29株中的8株（28%）有协同作用。每种抗生素组合对所有剩余的细菌都显示出相加或无关的作用。环丙沙星的协同作用代表了允许减少可能潜在地造成损害或副作用的相关药物（例如氨基糖苷类或β-内酰胺类）的剂量的重要优势。
The potential in vitro synergistic effect of ciprofloxacin associated with mezlocillin, cefoxitin and clindamycin was evaluated against 30 strains of Bacteroides fragilis. All bacteria were clinical isolates, identified by gas chromatography and fermentation of carbohydrates. MICs of the drugs were determined by a miniaturized dilution broth method. Resistance or susceptibility to the drugs was not a criterion for the selection of the strains. Antibacterial combinations were tested by a checkerboard method utilizing brain hearth infusion broth and a bacterial inoculum of 10(6) CFU/ml. Microtitre plates were incubated at 37 degrees C for 48 h in anaerobic jars (BBL). Prereduced chopped meat carbohydrate broth was used to store and culture the strains. The effect of antimicrobial combinations was defined on the basis of the fractionary inhibitory concentration (FIC) indexes. We considered: synergistic effect FIC less than or equal to 0.5; additive effect FIC = 0.5-0.75; indifferent effect FIC = 0.76-2.0; antagonism FIC greater than or equal to 2. Ciprofloxacin + clindamycin showed synergistic activity against 10 strains out of 26 (38%); ciprofloxacin + mezlocillin against 9 strains out of 30 (30%); and ciprofloxacin + cefoxitin against 8 out of 29 strains (28%). Each antibiotic combination showed an additive or indifferent effect against all remaining bacteria. The synergistic effect of ciprofloxacin represents an important advantage in allowing the reduction of the dosage of associated drugs such as aminoglycosides or beta-lactams that can potentially be responsible for damage or side-effects.