Inoculants of leguminous crops for mitigating soil emissions of the greenhouse gas nitrous oxide
Inoculants of leguminous crops for mitigating soil emissions of the greenhouse gas nitrous oxide
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DOI：
10.1007/s11104-011-0820-0
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发表时间：
2011-09-01
期刊：
影响因子：
4.9
通讯作者：
Revellin, Cecile
中科院分区：
文献类型：
--
作者：
Henault, Catherine;Revellin, Cecile
一氧化二氮(N2O)是一种温室气体，也是造成臭氧消耗的原因之一，主要来源于土壤和农业活动。我们研究了携带nosZ基因的慢生根瘤菌接种剂对土壤N2O排放的影响。在温室盆栽接种大豆植株上，研究了慢生根瘤菌(USDA110和MSDJ G49)对N2O的消耗，并在实验室条件下研究了不同氧浓度和N2O浓度梯度下接种大豆根瘤对N2O的消耗。在温室试验中，我们从N2O源系统(土壤+接种nosZ基因缺失菌株的大豆)转换为N2O汇系统(土壤+接种nosZ基因缺失菌株的大豆)。在好氧条件下，慢生根瘤菌USDA110和MSDJ G49均能还原N2O，其还原速率随N2O浓度的增加而增加。利用获得的定量结果进行的计算清楚地表明，这一过程在现场范围内具有环境效益。这项研究表明，在豆科作物上接种根瘤菌是减少耕作土壤N2O排放的一个有前途的领域，还需要进一步的研究才能最好地评估数量效益。
Nitrous oxide (N2O) is a greenhouse gas which is also responsible for ozone depletion, that mainly originates from soils and agricultural activities. We investigated the ability of inoculants of Bradyrhizobium japonicum carrying the nosZ gene to mitigate soil N2O emissions. The consumption of N2O by strains of Bradyrhizobium japonicum (USDA110 and MSDJ G49) was investigated both on inoculated soybean plants cultivated in soil pots during a greenhouse experiment and on detached nodules submitted to gradients of oxygen and N2O concentrations in laboratory conditions. During the greenhouse experiment, we switched from a system acting as an N2O source (soil + soybean inoculated with a nosZ gene depleted strain) to a system acting as an N2O sink (soil + soybean inoculated with strains carrying the nosZ gene). Nodules of Bradyrhizobium japonicum USDA110 and MSDJ G49 were both able to reduce N2O under aerobic conditions at rates increasing with N2O concentrations. Calculations using the obtained quantitative results clearly suggest an environmental benefit of this process on the field scale. This study demonstrates that the inoculation of rhizobia strains on leguminous crops is a promising area for mitigating N2O emission by cultivated soils and that further researches are required to best evaluate quantitative benefits.