GBS Data Identify Pigmentation-Specific Genes of Potential Role in Skin-Photosensitization in Two Tunisian Sheep Breeds
GBS Data Identify Pigmentation-Specific Genes of Potential Role in Skin-Photosensitization in Two Tunisian Sheep Breeds
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DOI：
10.3390/ani10010005
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发表时间：
2020-01-01
期刊：
影响因子：
3
通讯作者：
Bedhiaf-Romdhani, Sonia
中科院分区：
文献类型：
--
作者：
Baazaoui, Imen;McEwan, John;Bedhiaf-Romdhani, Sonia
简单总结 保持本土动物遗传资源的灵活性以应对当地环境限制仍然是一项重大挑战。在突尼斯，Noire de Thibar 品种是当地的一种绵羊，通常呈黑色，以其耐受“贯叶连翘”的能力而闻名，这种现象会导致白色绵羊的皮肤光敏化。本研究的目标是进行基因组扫描，通过考虑在不同毛色绵羊（黑色与白色）中进行基因分型的测序基因分型（GBS）标记，以识别参与皮肤光敏化的强且最新的人工选择。有趣的是，基因组分化分析确定了基因组区域内包含关键色素沉着和光敏性相关基因的 F-ST 标记。这些发现有助于了解毛色遗传学的背景及其在适应当地环境限制中的潜在作用。 摘要 突尼斯 Noire de Thibar 绵羊品种是一个复合品种，最近选择培育出全身黑色的动物，以避免摄入有毒的“贯叶连翘”杂草后出现皮肤光敏反应，从而给养羊户造成重大经济损失。我们使用黑色绵羊 (Noire de Thibar) 和相关白毛绵羊 (Queue Fine de l'ouest) 品种的基因分型测序 (GBS) 数据评估遗传分化并估计标记 F-ST，以识别人工选择的信号。结果揭示了与毛色相关的候选基因中的选择特征，这些特征被认为间接参与绵羊的光敏机制。鉴定出的基因可以为分子育种提供重要信息。
Simple Summary Maintaining the flexibility of the genetic resources of native animals to face local environment constraints is still a major challenge. In Tunisia, the Noire de Thibar breed is a local sheep, typically with black coloration, known for its ability to tolerate "hypericum perforatum", which causes skin photosensitization in white colored sheep. The goal of this study was to perform a genome scan, by considering genotyping-by-sequencing (GBS) markers that were genotyped in divergent coat colored sheep (black vs. white) to identify strong, and recent, artificial selection that is involved in skin-photosensitization. Interestingly, the genomic differentiation analysis identified F-ST markers within genomic regions containing key pigmentation and photosensitivity related-genes. These findings help in understanding the background of coat color genetics and its potential role in adaptation to local environment constraints.Abstract The Tunisian Noire de Thibar sheep breed is a composite breed, recently selected to create animals that are uniformly black in order to avoid skin photosensitization after the ingestion of toxic "hypericum perforatum" weeds, which causes a major economic loss to sheep farmers. We assessed genetic differentiation and estimated marker F-ST using genotyping-by-sequencing (GBS) data in black (Noire de Thibar) and related white-coated (Queue fine de l'ouest) sheep breeds to identify signals of artificial selection. The results revealed the selection signatures within candidate genes related to coat color, which are assumed to be indirectly involved in the mechanism of photosensitization in sheep. The identified genes could provide important information for molecular breeding.