Triphenyltin acetate-induced cytotoxicity and CD4+ and CD8+ depletion in mouse thymocyte primary cultures
Triphenyltin acetate-induced cytotoxicity and CD4+ and CD8+ depletion in mouse thymocyte primary cultures
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DOI：
10.1016/s0300-483x(01)00520-0
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发表时间：
2001-12-28
期刊：
影响因子：
4.5
通讯作者：
Bollo, E
中科院分区：
文献类型：
--
作者：
Dacasto, M;Cornaglia, E;Bollo, E
有机锡化合物（OT）在全球范围内用作催化剂、稳定剂和杀菌剂。三苯基锡衍生物是我国使用最多的有机锡类杀菌剂，包括杀菌剂三苯基锡乙酸酯（TPTA）。本文用原代培养的小鼠胸腺细胞研究了TPTA的细胞毒性、对特定淋巴细胞亚群的选择活性以及抗增殖作用。TPTA（5、10和25 μ M）对这些细胞具有细胞毒性，如通过细胞活力百分比（台盼蓝染料排除试验）、中性红摄取和四唑盐还原为甲瓒产物（MTT测定）的显著（P < 0.05）降低所证明的。在暴露于TPTA 4小时后，已经注意到这些明显的影响。除此之外，在孵育24小时后，杀真菌剂显著降低了成熟的单阳性胸腺细胞的百分比，特别是CD 4（+）/CD 8（-）胸腺细胞的百分比。最后，在暴露于1和8 μ M TPTA的胸腺细胞中观察到T细胞有丝分裂原诱导的细胞增殖的显著剂量依赖性抑制。这些结果表明TPTA具有免疫毒性。根据先前发表的关于某些二-和三有机锡化合物的体外和体内毒性的报道。(C)2001爱思唯尔科学爱尔兰有限公司保留所有权利。
Organotin compounds (OTs) find application worldwide as catalysts, stabilizers and biocides. Triphenyltin derivatives (TPs), including the fungicide triphenyltin acetate (TPTA), are OTs mostly used in our country. Some OTs were proved to be immunotoxic and in this paper the cytotoxicity, the possible selective activity upon definite lymphocyte subsets as well as the antiproliferative effect of TPTA was investigated in vitro by using primary cultures of mouse thymocytes. TPTA (5, 10 and 25 muM) was cytotoxic to these cells, as demonstrated by the significant (P < 0.05) reduction of the cell viability percentage (trypan blue dye exclusion test), the neutral red uptake and the reduction of tetrazolium salts to formazan products (MTT assay). These overt effects were already noticed after 4 h of exposure to TPTA. The fungicide otherwise significantly reduced, after 24 h of incubation, the percentage of mature single positive thymocytes, particularly the CD4(+)/CD8(-) one. Finally, a significative dose-dependent inhibition of the T-cell mitogen-induced cell proliferation was observed in thymocytes exposed to l and 8 muM TPTA. These results are indicative of the TPTA immunotoxic properties. according to previous published reports concerning the in vitro and in vivo toxicity of some di- and triorganotin compounds. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.