Proteomics quantifies protein expression changes in a model cnidarian colonised by a thermally tolerant but suboptimal symbiont
Proteomics quantifies protein expression changes in a model cnidarian colonised by a thermally tolerant but suboptimal symbiont
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DOI：
10.1038/s41396-019-0437-5
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发表时间：
2019-05
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影响因子：
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通讯作者：
Ashley E Sproles;Clinton A. Oakley;J. Matthews;Lifeng Peng;J. Owen;A. Grossman;V. Weis;S. Davy
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文献类型：
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作者：
Ashley E Sproles;Clinton A. Oakley;J. Matthews;Lifeng Peng;J. Owen;A. Grossman;V. Weis;S. Davy
珊瑚获取耐热藻类共生体被认为是提高珊瑚礁对人为气候变化适应能力的一种自然或辅助机制，但决定新共生组合表现的细胞水平过程却知之甚少。我们用液相色谱-质谱仪研究了实验诱导的共生对模式海葵Exaiptasia pallida寄主蛋白质组的影响。非共生的标本被同源甲藻共生体(BreVium Minutom)或耐热的、生态入侵的异源共生体(Durusddium Trenchii)定殖。海葵含有D。Trenchii显示出最低限度的Niemann-Pick C2蛋白和谷氨酰胺合成酶的表达，Niemann-Pick C2蛋白和谷氨酰胺合成酶被认为参与伴侣之间的氮同化和再循环。Niemann-Pick C2蛋白预测了固醇运输和细胞识别的生化作用。与非共生处理相比，两种共生处理中多个溶酶体相关蛋白的含量都较低。这些差异丰富的蛋白质被预测为可能参与伙伴之间的营养运输或资源分配的途径。这些结果为阐明珊瑚-甲藻共生中补偿生理的机制提供了具体的实验靶标。
The acquisition of thermally tolerant algal symbionts by corals has been proposed as a natural or assisted mechanism of increasing coral reef resilience to anthropogenic climate change, but the cell-level processes determining the performance of new symbiotic associations are poorly understood. We used liquid chromatography–mass spectrometry to investigate the effects of an experimentally induced symbiosis on the host proteome of the model sea anemoneExaiptasia pallida. Aposymbiotic specimens were colonised by either the homologous dinoflagellate symbiont (Breviolum minutum) or a thermally tolerant, ecologically invasive heterologous symbiont (Durusdinium trenchii). Anemones containingD. trenchiiexhibited minimal expression of Niemann-Pick C2 proteins, which have predicted biochemical roles in sterol transport and cell recognition, and glutamine synthetases, which are thought to be involved in nitrogen assimilation and recycling between partners.D. trenchii-colonised anemones had higher expression of methionine-synthesising betaine–homocysteine S-methyltransferases and proteins with predicted oxidative stress response functions. Multiple lysosome-associated proteins were less abundant in both symbiotic treatments compared with the aposymbiotic treatment. The differentially abundant proteins are predicted to represent pathways that may be involved in nutrient transport or resource allocation between partners. These results provide targets for specific experiments to elucidate the mechanisms underpinning compensatory physiology in the coral–dinoflagellate symbiosis.