MODIFICATION OF CELL-MEMBRANES WITH VIRAL ENVELOPES DURING FUSION OF CELLS WITH HVJ (SENDAI VIRUS) .1. INTERACTION BETWEEN CELL-MEMBRANES AND VIRUS IN EARLY STAGE
MODIFICATION OF CELL-MEMBRANES WITH VIRAL ENVELOPES DURING FUSION OF CELLS WITH HVJ (SENDAI VIRUS) .1. INTERACTION BETWEEN CELL-MEMBRANES AND VIRUS IN EARLY STAGE
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DOI：
10.1016/0014-4827(75)90462-0
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发表时间：
1975-01-01
影响因子：
3.7
通讯作者：
MATSUI, Y
中科院分区：
文献类型：
--
作者：
OKADA, Y;KOSEKI, I;MATSUI, Y
细胞与HVJ在37℃下融合30 min后，大量病毒物质与细胞表面相关。这是由于病毒包膜与细胞膜的融合。研究了从病毒吸附到细胞-细胞或细胞-病毒包膜融合的过程。在低温（例如0-15°C）孵育时，虽然病毒和细胞之间有一些相互作用，但未观察到包膜融合或细胞融合。这种相互作用导致细胞血氧吸附（HA）活性的丧失和细胞膜离子屏障的部分损伤。病毒颗粒似乎在低温下接近细胞膜的脂质层，扭曲了非柔性的膜结构。细胞-病毒复合物在37℃孵育后，细胞迅速变成HA阳性，HA活性在5 min内达到最大值。在这个阶段，有许多离子通过细胞膜渗漏。进一步孵育后，细胞膜离子屏障损伤完全修复，细胞融合完成。这一过程可能与病毒包膜与细胞膜的融合以及与之融合的细胞膜的恢复有关。
A large number of viral materials are associated with the surface of cells after cell fusion with HVJ at 37 °C for 30 min. This is due to fusion of viral envelopes with the cell membrane. Studies were made on the process from viral adsorption to cell-cell, or cell-viral envelope fusion. On incubation at low temperatures, such as 0–15 °C, no envelope fusion or cell fusion was observed, although there was some interaction between the virus and cells. This interaction resulted in loss of hemadsorption (HA) activity of the cells and partial damage of the ion barrier of the cell membrane. The viral particles seem to come close to the lipid layer of the cell membrane at the low temperatures and to distort the non-flexible membrane structure. On incubation of the cell-virus complex at 37 °C, the cells rapidly became HA-positive and the HA activity was maximal within 5 min. At this stage there was much leakage of ions through the cell membrane. On further incubation the damage to the ion barrier of the cell membrane was repaired completely with completion of cell fusion. This process may be correlated with fusion of viral envelopes with cell membranes and restoration of the cell membrane fused with them.