MOLECULAR-CLONING OF A CDNA SEQUENCE ENCODING A TROPHECTODERM-SPECIFIC MARKER DURING MOUSE BLASTOCYST FORMATION
MOLECULAR-CLONING OF A CDNA SEQUENCE ENCODING A TROPHECTODERM-SPECIFIC MARKER DURING MOUSE BLASTOCYST FORMATION
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DOI：
10.1073/pnas.79.7.2328
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发表时间：
1982-01-01
期刊：
影响因子：
--
通讯作者：
JACOB, F
中科院分区：
文献类型：
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作者：
BRULET, P;JACOB, F
从滋养细胞瘤基因文库中分离到一个c[互补]DNA克隆。与该序列互补的信使核糖核酸指导蛋白质的体外合成。这些蛋白的二维迁移模式与单抗Troma-1识别的滋养细胞瘤中间丝蛋白的双向迁移模式是重叠的。该单抗标记滋养外胚层细胞，但不标记内细胞团细胞。通过灵敏的结合试验(超灵敏的酶免疫分析)，这些体外合成的蛋白可被mAbTroma-1识别。这些蛋白是由针对滋养细胞瘤中间丝蛋白的抗血清和针对在小鼠胚胎外内皮细胞系(Endo-A)中发现的主要细胞骨架蛋白的血清免疫沉淀的。该序列在滋养细胞瘤中检测到与18SRRNA一起迁移的特异性基因(S)，而在胚胎癌细胞中检测不到。
A c[complementary]DNA clone was isolated from a trophoblastoma cDNA library. mRNA complementary to this sequence directs the in vitro synthesis of proteins. The 2-dimensional electrophoretic pattern of migration of these proteins is superposable to that of the trophoblastoma intermediate filament proteins recognized by monoclonal antibody (mAb) TROMA-1. This mAb labeled trophectoderm cells but not inner cell mass cells. With a sensitive binding assay (ultrasensitive enzymatic radioimmunoassay), these in vitro synthesized proteins were recognized by mAb TROMA-1. The proteins are immunoprecipitated by an antiserum directed against trophoblastoma intermediate filament proteins and by a serum directed against a major cytoskeletal protein found in murine extraembryonic endodermal cell lines (Endo-A). The cDNA sequence detects specific mRNA(s) migrating with 18S rRNA in trophoblastoma but not in embryonal carcinoma cells.