Transgenically expressed rice germin-like protein1 in tobacco causes hyper-accumulation of H2O2 and reinforcement of the cell wall components
Transgenically expressed rice germin-like protein1 in tobacco causes hyper-accumulation of H2O2 and reinforcement of the cell wall components
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DOI：
10.1016/j.bbrc.2010.10.073
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发表时间：
2010-11-26
影响因子：
3.1
通讯作者：
Maiti, Mrinal K.
中科院分区：
文献类型：
--
作者：
Banerjee, Joydeep;Das, Natasha;Maiti, Mrinal K.
我们最近的报道表明，水稻芽状蛋白1 （OsGLP1）是一种细胞壁相关蛋白，参与水稻抗病，并具有烟草中异源表达的超氧化物歧化酶（SOD）活性。本研究进一步分析了转基因烟草植株，以揭示OsGLP1及其相关SOD活性的详细生理生化功能组织学染色显示转基因植株茎组织中细胞壁成分交联增强，傅里叶变换红外光谱（FTIR）对转基因植株茎组织中的生物聚合物进行分析，发现转基因植株与未转化植株茎组织中的生物聚合物存在差异我们的研究结果表明，具有固有SOD活性的OsGLP1负责H2O2的超积累和细胞壁成分的增强(C) 2010 Elsevier Inc . All rights reserved
Our recent report documented that the rice germin-like protein1 (OsGLP1) being a cell wall-associated protein involves in disease resistance in rice and possesses superoxide dismutase (SOD) activity as recognized by heterologous expression in tobacco In the present study the transgenic tobacco plants were analyzed further to decipher the detailed physiological and biochemical functions of the OsGLP1 and its associated SOD activity The transgenic tobacco lines expressing SOD-active OsGLP1 showed tolerance against biotic and abiotic stresses mitigated by hyper-accumulating H2O2 upon infection by fungal pathogen (Fusarium solani) and treatment to chemical oxidizing agent (ammonium persulfate) respectively Histological staining revealed enhanced cross-linking of the cell wall components in the stem tissues of the transgenic plants Fourier transform infrared spectroscopy (FTIR) analysis of the biopolymer from the stem tissues of the transgenic and untransformed plants revealed differential banding pattern of the spectra corresponding to various functional groups Our findings demonstrate that the OsGLP1 with its inherent SOD activity is responsible for hyper-accumulation of H2O2 and reinforcement of the cell wall components (C) 2010 Elsevier Inc All rights reserved