A novel tetrasaccharide, with a structure similar to the terminal sequence of an arabinogalactan-protein, accumulates in rice anthers in a stage-specific manner
A novel tetrasaccharide, with a structure similar to the terminal sequence of an arabinogalactan-protein, accumulates in rice anthers in a stage-specific manner
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DOI：
10.1046/j.1365-313x.1996.9060777.x
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发表时间：
1996-06-01
期刊：
影响因子：
7.2
通讯作者：
Ishii, T
中科院分区：
文献类型：
--
作者：
Kawaguchi, K;Shibuya, N;Ishii, T
利用高效阴离子交换色谱（HPAEC）对水稻花药中的游离糖进行了分析，发现在小孢子形成过程中积累了一种高浓度的新型低聚糖。通过甲基化分析、质谱/质谱（MS/MS）和核磁共振（NMR）对纯化的低聚糖进行了结构分析，结果表明其结构为β - l - ara (f)-(1—bbb3)- α - l - ara (f)-(1—bbb3)- β - d - gal (p)-(1—bbb6)- d - gal，与植物细胞表面蛋白聚糖阿拉伯半乳糖蛋白（AGP）聚糖链中的一个四糖单元密切相关。低温处理（12℃，4 d）在水稻小孢子形成过程中对花药造成损伤，使四糖含量降低，而蔗糖含量升高。这种效应在对低温敏感的突变系YM56-1中尤为明显。这些结果表明，这种独特的四糖可能在水稻花药的发育及其对低温的反应中起重要作用。
Analysis of free sugars in developing rice anthers by high-performance anion-exchange chromatography (HPAEC) showed that a very high concentration of a novel oligosaccharide accumulated specifically during microsporogenesis. Structural analysis of the purified oligosaccharide by methylation analysis, mass spectrometry/mass spectrometry (MS/MS), and nuclear magnetic resonance (NMR) spectroscopy revealed its structure to be beta-L-Ara(f)-(1-->3)-alpha-L-Ara(f)-(1-->3)-beta-D-Gal(p)-(1-->6)-D-Gal, which is closely related to a tetrasaccharide unit found in the glycan chain of a plant cell surface proteoglycan, the arabinogalactan-protein (AGP). Chilling treatment (12 degrees C, 4 days), which injures rice anthers during microsporogenesis, decreased the concentration of the tetrasaccharide, but the sucrose level increased. This effect was especially evident in a chilling-sensitive mutant line, YM56-1. These results suggest that this unique tetrasaccharide may play an important role in both the development of the rice anther and its response to chilling.