Chinese spring wheat (Triticum aestivum L.) chloroplast genome:: Complete sequence and contig clones
Chinese spring wheat (Triticum aestivum L.) chloroplast genome:: Complete sequence and contig clones
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DOI：
10.1007/bf02823995
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发表时间：
2000-01-01
影响因子：
2.1
通讯作者：
Tsunewaki, K
中科院分区：
文献类型：
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作者：
Ogihara, Y;Isono, K;Tsunewaki, K
覆盖普通小麦（Triticum aestivum cv.）整个叶绿体（cp）基因组的质粒克隆文库。中国春构建并组装成重叠群克隆。由此，我们获得了小麦叶绿体DNA的完整核苷酸序列-一个134，540 bp的环状DNA（DDBJ登录号AB 042240），包含4种核糖体RNA、20种转运RNA的30个基因和71个蛋白质编码基因。此外，我们检测到五个未识别的开放阅读框架之间保存的草。可根据要求提供质粒克隆。
Libraries of plasmid clones covering the entire chloroplast (cp) genome of the common wheat, Triticum aestivum cv. Chinese Spring were constructed and assembled into contig-clones. From these, we obtained the complete nucleotide sequence of wheat chloroplast DNA - a 134,540 bp circular DNA (DDBJ accession no. AB042240) containing four species of ribosomal RNA, 30 genes for 20 species of transfer RNA, and 71 protein coding genes. Additionally, we detected five unidentified open reading frames conserved among grasses. Plasmid clones are available on request.