Synthetic mimics of biotin/(strept)avidin.
Synthetic mimics of biotin/(strept)avidin.
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DOI：
10.1039/c7cs00011a
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发表时间：
2017-05-09
影响因子：
46.2
通讯作者：
Isaacs L
中科院分区：
文献类型：
--
作者：
Liu W;Samanta SK;Smith BD;Isaacs L
生物素/亲和素自组装是纳米级制造和捕获的强大平台，在科学，医学和纳米技术中有许多不同的应用。然而，生物素/亲和素自组装有几个众所周知的缺点，限制了某些技术领域的性能，并且需要合成模拟物，这些模拟物可以成为更好的替代品或具有生物正交识别特性的操作伙伴。本教程综述的目的是描述在水或生物介质中操作的高亲和合成关联伙伴的最新进展。本文首先概述了生物素/(strept)亲和素自组装的背景和目前创建合成模拟物的设计规则。介绍了一系列的案例研究，描述了最近成功使用合成衍生物环糊精，葫芦烷和各种有机环烷，如杯芳烃，深空腔体，柱芳烃和四内酰胺。在某些情况下，两个互补的伙伴结合产生纳米级复合物，在其他情况下，一个异位宿主分子被用来连接两个伙伴。文章最后简要讨论了未来的发展方向和可能面临的挑战。
Biotin/(strept)avidin self-assembly is a powerful platform for nanoscale fabrication and capture with many different applications in science, medicine, and nanotechnology. However, biotin/(strept)avidin self-assembly has several well-recognized drawbacks that limit performance in certain technical areas and there is a need for synthetic mimics that can either become superior replacements or operational partners with bio-orthogonal recognition properties. The goal of this tutorial review is to describe the recent progress in making high affinity synthetic association partners that operate in water or biological media. The review starts with a background summary of biotin/(strept)avidin self-assembly and the current design rules for creating synthetic mimics. A series of case studies are presented that describe recent success using synthetic derivatives of cyclodextrins, cucurbiturils, and various organic cyclophanes such as calixarenes, deep cavitands, pillararenes, and tetralactams. In some cases, two complementary partners associate to produce a nanoscale complex and in other cases a ditopic host molecule is used to link two partners. The article concludes with a short discussion of future directions and likely challenges.