Integrated microfluidic system with electrochemically actuated on-chip pumps and valves
Integrated microfluidic system with electrochemically actuated on-chip pumps and valves
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DOI：
10.1016/s0925-4005(03)00482-9
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发表时间：
2003-11-15
影响因子：
8.4
通讯作者：
Yoneyama, R
中科院分区：
文献类型：
--
作者：
Suzuki, H;Yoneyama, R
微流控系统的构建是通过集成芯片上的微型泵和止回阀，其功能通过电化学产生或熄灭的氢气泡。基本元件由薄膜三电极系统组成，包括铂黑工作电极。在聚二甲基硅氧烷（PDMS）基底上形成用于电解质溶液的微流动通道和容器。通过将工作电极电位设定为恒定值，可再现地控制气泡的生长和收缩。堵塞在流动通道中间的阀室中的气泡的弹性性质阻碍了溶液的有效通过。气泡在分离两种不同的溶液时也是有效的。通过使阀协同地打开和关闭，溶液可以被引入到系统中并在流动通道中输送。当两种染料溶液在流动通道中运输和合并时，观察到鞘流，反映了低雷诺数。作为模型系统，分别引入、运输和混合含有荧光素酶和荧光素酶的两种溶液。观察到源自酶促反应的化学发光。(C)2003 Elsevier Science B.V.保留所有权利。
A microfluidic system was constructed by integrating on-chip micropumps and check valves that function by means of a hydrogen bubble that is generated or extinguished electrochemically. Essential elements consisted of thin-film three-electrode systems, including a platinum black working electrode. Micro flow channels and containers for electrolyte solutions were formed on a polydimethylsilixane (PDMS) substrate. The growth and shrinkage of the bubble were controlled reproducibly by setting the working electrode potential at a constant value. The elastic nature of the bubble clogged in a valve compartment in the middle of the flow channel hindered the effective passage of the solution. The bubble was also effective in separating two different solutions. By making the valves open and close cooperatively, a solution could be introduced into the system and transported in the flow channel. When two dye solutions were transported and merged in a flow channel, sheath flows were observed, reflecting a low Reynolds number. As a model system, two solutions containing luciferin and luciferase were introduced separately, transported, and mixed. Chemiluminescence originating from the enzymatic reaction was observed. (C) 2003 Elsevier Science B.V. All rights reserved.