A new magnetic bead-based, filterless bio-separator with planar electromagnet surfaces for integrated bio-detection systems
A new magnetic bead-based, filterless bio-separator with planar electromagnet surfaces for integrated bio-detection systems
复制标题
DOI：
10.1016/s0925-4005(00)00458-5
复制
发表时间：
2000-08-25
影响因子：
8.4
通讯作者：
Henderson, HT
中科院分区：
文献类型：
--
作者：
Choi, JW;Ahn, CH;Henderson, HT
设计、制造了一种从载液中分离磁性微珠的新型无过滤生物分离器，并将其表征为生物细胞采样和检测系统的核心部件。为了最大限度地提高采样能力，实现了带有蛇形线圈和半封装坡莫合金的平面电磁体表面。利用该生物分离器，成功地将包被抗体的磁珠从生物缓冲悬浮液中分离出来，并在动态流体中进行了表征。对生物分离器的磁特性进行了模拟和实验研究，建立并验证了生物分离器的制造设计准则。所实现的无滤生物分离器在生物医学和生物检测系统中具有很高的应用前景。它特别适用于芯片上微全分析系统(MU-TAS)或远程检测系统中对少量生物分子(如抗原)的选择性采样和分离。(C)2000 Elsevier Science S.A.保留所有权利。
A new filterless bio-separator separating magnetic microbeads from a carrier fluid has been designed, fabricated, and characterized as a core component of biological cell sampling and detecting systems. To maximize the sampling capability, a planar electromagnet surface with a serpentine coil and semi-encapsulated permalloy has been realized. Using this bio-separator, antibody-coated magnetic beads have been successfully separated from the bio-buffer suspension solution and characterized in dynamic fluid flow. The magnetic characteristics of the bio-separator have been simulated and experimentally studied to establish and validate the design rules for fabrication. The realized filterless bio-separator has a high potential in biomedical and biological detection systems. It is especially useful in selective sampling and separation of small amounts of bio-molecules (e.g., antigen) for on-chip micro total analysis systems (mu-TAS) or remote detection systems. (C) 2000 Elsevier Science S.A. All rights reserved.