NT-4/5 REDUCES NATURALLY-OCCURRING RETINAL GANGLION-CELL DEATH IN NEONATAL RATS
NT-4/5 REDUCES NATURALLY-OCCURRING RETINAL GANGLION-CELL DEATH IN NEONATAL RATS
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DOI：
10.1097/00001756-199410000-00010
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发表时间：
1994-10-03
期刊：
影响因子：
1.7
通讯作者：
HARVEY, AR
中科院分区：
文献类型：
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作者：
CUI, Q;HARVEY, AR
将逆行亲核示踪剂二胺黄（DY）注射于2日龄大鼠左上丘。2天后，右眼注射神经营养因子NT-4/5、环己亚胺（CHX）、MK-801和DNQX（谷氨酸受体拮抗剂）或生理盐水。几乎所有的大鼠在6-7 h后灌注，并在视网膜全坐位上检测正常和收缩的dy标记视网膜神经节细胞（RGCs）的数量。在对照组（无眼注射）中，收缩性RGCs比例为1.04%。注射NT-4/5后，自然死亡水平显著降低（0.34%）；正常RGC密度也较高。注射生理盐水（1.21%）、MK-801/DNQX（1.22%）或CHX（1.48%）后，RGC固缩增加，但只有后者的增加明显大于对照组。
THE retrograde nucleophilic tracer diamidino yellow (DY) was injected into the left superior colliculi of 2-day-old rats. Two days later, right eyes were injected with either neurotrophin NT-4/5, cycloheximide (CHX), MK-801 and DNQX (glutamate receptor antagonists), or saline. Almost all rats were perfused 6-7 h later and the numbers of normal and pyknotic DY-labelled retinal ganglion cells (RGCs) were determined from retinal whole mounts. In controls (no eye injection) the proportion of pyknotic RGCs was 1.04%. This level of naturally occurring death was significantly reduced after injection of NT-4/5 (0.34%); normal RGC density was also higher in this group. RGC pyknosis was increased after saline (1.21%), MK-801/DNQX (1.22%) or CHX (1.48%) injections but only in the latter case was the increase significantly greater than control.