Simple method for determination of oxalic acid in forages using high‐performance liquid chromatography
Simple method for determination of oxalic acid in forages using high‐performance liquid chromatography
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高效液相色谱法测定饲料中草酸的简易方法
DOI：
10.1111/j.1744-697x.2007.00093.x
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发表时间：
2007
影响因子：
1.3
通讯作者：
O. Kawamura
中科院分区：
文献类型：
--
作者：
M. M. Rahman;M. Niimi;O. Kawamura
建立了以阴离子分析柱（Shodex 1C SI-90 4E）和碳酸氢钠（NaHCO3）为洗脱液，高效液相色谱法测定牧草中草酸盐含量的简便方法。用水或1 mol HCl提取草酸（分别为可溶性形式或总形式）后测定草酸。不溶草酸是总草酸和可溶草酸之间的差值。高效液相色谱法测定草酸回收率达97%以上。该方法可成功测定牧草中的草酸盐。该方法简便、快速、经济。
We established a simple method for determination of oxalate in forage grasses using high-performance liquid chromatography (HPLC) with an anion analysis column (Shodex 1C SI-90 4E) and sodium hydrogen carbonate (NaHCO3) as eluent. Oxalate was determined after extraction with water or 1 mol HCl (soluble form or total, respectively). Insoluble oxalate was estimated as the difference between total oxalates and soluble form. Recovery of oxalate after HPLC was above 97%. Oxalates in forages could be successfully determined by this method. The method was simple, fast and inexpensive.