Anti-allergic properties of Cissampelos sympodialis and its isolated alkaloid warifteine
Anti-allergic properties of Cissampelos sympodialis and its isolated alkaloid warifteine
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DOI：
10.1016/j.intimp.2006.02.007
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发表时间：
2006-07-01
影响因子：
5.6
通讯作者：
Bozza, Patricia T.
中科院分区：
文献类型：
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作者：
Bezerra-Santos, Claudio R.;Vieira-de-Abreu, Adriana;Bozza, Patricia T.
能够调节嗜酸性炎症的新药物的开发可以发现治疗过敏性疾病的新方法，如哮喘。在这里，我们评估了巴西防己科植物三叶草提取物的抗过敏特性，重点是它对过敏性嗜酸性粒细胞增多症的影响。通过对主动致敏Balb/c小鼠哮喘和过敏性胸膜炎两种变态反应性炎症模型的研究，我们观察到C sympodiis口服液可剂量依赖性地减少过敏原激发的胸膜嗜酸性粒细胞内流。C蛋白的抑制作用与嗜酸性粒细胞运动机制无关，但依赖于嗜酸性粒细胞趋化因子的抑制作用。嗜酸性粒细胞趋化因子在过敏反应中起重要作用。中草药还能影响嗜酸性粒细胞的活化，抑制新的细胞质脂体的形成和半胱氨酰白三烯的分泌。从C sympodiis提取物中分离出的生物碱Warifteine是该提取物抗嗜酸粒细胞作用的活性成分，因为Warifteine能够重现C sympodiis对过敏性嗜酸性粒细胞增多症和半胱氨基白三烯产生的抑制作用。值得注意的是，C sympodiis和Warifteine的后处理也有效地抑制了过敏攻击后观察到的嗜酸性反应。因此，C sympodiis/warifteine可能是一种很有前途的抗过敏药物，因为它具有强大的抗嗜中性粒细胞和抗白三烯活性。(C)2006爱思唯尔B.V.保留所有权利。
Development of new agents capable of regulating eosinophilic inflammation can uncover novel therapeutic approaches for the treatment of allergic diseases, such as asthma. Here, we evaluated the anti-allergic properties of an extract of the Brazilian Menispermaceae Cissampelos sympodialis, focusing on its effects on allergic eosinophilia. By studying two models of allergic inflammation, an asthma model and the allergic pleurisy in actively sensitized Balb/c mice, we observed that the oral pre-treatment with C sympodialis reduced pleural eosinophil influx triggered by allergen challenge in a dose-dependent manner. The mechanism involved in C sympodialis inhibitory effect appeared to be independent of a direct effect on eosinophil locomotory machinery, but depend on a blockage of eotaxin production, a key eosinophil chemoattractant with important roles in allergic reactions. C sympodialis was also able to affect eosinophil activation, as attested by its ability of inhibiting formation of new cytoplasmic lipid bodies and the secretion of cysteinyl leukotrienes. The alkaloid warifteine isolated from the C sympodialis extract represents an active component responsible for the anti-eosinophilic effects of the extract, since warifteine was able to reproduce C sympodialis inhibitory effects on allergic eosinophilia and cysteinyl leukotrienes production. Of interest, C sympodialis and warifteine post-treatments also effectively inhibited eosinophilic reaction observed after allergic challenge. Therefore, C sympodialis/warifteine may be a promising anti-allergic therapy, inasmuch as it presents potent anti-cosinophil and anti-leukotrienes activities. (c) 2006 Elsevier B.V. All rights reserved.