Disturbing effect of cationic amphiphilic drugs on phospholipid asymmetry of the membrane lipid bilayer of human erythrocytes.
Disturbing effect of cationic amphiphilic drugs on phospholipid asymmetry of the membrane lipid bilayer of human erythrocytes.
复制标题
阳离子两亲性药物对人红细胞膜脂双层磷脂不对称性的干扰作用。
DOI：
10.1248/cpb.31.1692
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发表时间：
1983
影响因子：
1.7
通讯作者：
T. Fujii
中科院分区：
文献类型：
--
作者：
A. Tamura;N. Moriwaki;T. Fujii
在37°C下用阳离子两亲性药物（各种叔胺吩噻嗪、伯氨喹或正癸胺中的一种）处理人红细胞后，高达45%的膜磷脂酰乙醇胺（PE）被添加的来自蜂毒的磷脂酶A2降解（未处理的红细胞，1-2%），并且约35%的PE可以被三硝基苯磺酸盐标记（未处理的红细胞，约14%）。胰腺磷脂酶A2实际上不能水解完整红细胞的磷脂，可降解用上述药物预处理的红细胞膜中的大量磷脂酰胆碱和PE。这些结果表明，阳离子药物一般可以改变膜的蛋白质-脂质相互作用，从而释放内层磷脂PE从可能的位置限制下的某些蛋白质的影响。
After treatment of human erythrocytes at 37°C with a cationic amphiphilic drug (one of various tertiary amine phenothiazines, primaquine or n-decylamine), up to 45% of the membrane phosphatidylethanolamine (PE) was degraded by added phospholipase A2 from bee venom (untreated erythrocytes, 1-2%) and about 35% of the PE could be labelled by trinitrobenzenesulfonate (untreated erythrocytes, about 14%). Pancreatic phospholipase A2, which is virtually unable to hydrolyze the phospholipids of intact erythrocytes, degraded significant amounts of phosphatidylcholine and PE in the erythrocyte membrane pretreated with the above drugs. These results suggest that cationic drugs in general may alter the protein-lipid interaction of the membrane and thus release the inner layer phospholipid PE from possible locational restriction under the influence of certain proteins.