MALDI Mass Spectrometry Imaging of N-Linked Glycans in Tissues
MALDI Mass Spectrometry Imaging of N-Linked Glycans in Tissues
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DOI：
10.1007/978-981-13-2158-0_4
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发表时间：
2018-01-01
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影响因子：
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通讯作者：
Angel, Peggi M.
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文献类型：
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作者：
Drake, Richard R.;West, Connor A.;Angel, Peggi M.
基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）已被用于生物样品的聚糖成分分析二十年。MALDI质谱成像（MALDI-MSI）是一种适用于组织的方法，可以对单个薄组织切片内的数百至数千个分子进行高通量空间分析。使用MALDI-MSI分析组织内N-聚糖的能力是最近开发的方法，其允许鉴定和定位40个或更多个N-聚糖。关键组成部分是将肽-N-糖苷酶的分子涂层应用于组织，肽-N-糖苷酶是一种从蛋白质载体中释放N-聚糖的酶。在本章中，方法和途径，鲁棒性和再现性生成二维N-聚糖组织图谱的MALDI-MSI工作流程进行了总结。目前的优势和局限性的方法进行了讨论，以及潜在的未来应用的方法。
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been used for two decades to profile the glycan constituents of biological samples. An adaptation of the method to tissues, MALDI mass spectrometry imaging (MALDI-MSI), allows high-throughput spatial profiling of hundreds to thousands of molecules within a single thin tissue section. The ability to profile N-glycans within tissues using MALDI-MSI is a recently developed method that allows identification and localization of 40 or more N-glycans. The key component is to apply a molecular coating of peptide-N-glycosidase to tissues, an enzyme that releases N-glycans from their protein carrier. In this chapter, the methods and approaches to robustly and reproducibly generate two-dimensional N-glycan tissue maps by MALDI-MSI workflows are summarized. Current strengths and limitations of the approach are discussed, as well as potential future applications of the method.