A fiberless seed mutation in cotton is associated with lack of fiber cell initiation in ovule epidermis and alterations in sucrose synthase expression and carbon partitioning in developing seeds
A fiberless seed mutation in cotton is associated with lack of fiber cell initiation in ovule epidermis and alterations in sucrose synthase expression and carbon partitioning in developing seeds
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DOI：
10.1104/pp.118.2.399
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发表时间：
1998-10-01
期刊：
影响因子：
7.4
通讯作者：
Chourey, PS
中科院分区：
文献类型：
--
作者：
Ruan, YL;Chourey, PS
棉花（Gossypium hirsutum L.）胚珠代表了高等植物中毛状体发育的独特例子。关于控制这一过程的分子和代谢机制知之甚少。在这里，我们报告了一个无纤维种子（FLS）突变体（缺乏纤维）和正常（FLS）突变体的比较分析，以更好地了解纤维发育的初始细胞学事件，并分析与纤维素生物合成过程中蔗糖的主要汇的损失相关的代谢变化在突变种子。在开花当天（0 DAA），突变体胚珠表皮细胞缺乏典型的芽样突起，在FLS胚珠中看到的，并需要致力于纤维发育途径。细胞特异性基因表达分析表明，蔗糖合成酶（SuSy）的RNA和蛋白质在0 DAA检测不到的ils胚珠，但在丰富的，稳定的状态水平在启动纤维细胞的FLS胚珠。发育种子15至35 DAA的组织水平分析显示，相对于正常基因型的突变体中的SuSy表达的时间模式改变。SuSy表达的改变编程是突变的原因还是结果尚不清楚。与FLS基因型相比，FLS突变体发育中的种子也显示出几个相关的变化，这些变化代表了种皮和子叶中碳分配的改变。
Fiber cell initiation in the epidermal cells of cotton (Gossypium hirsutum L.) ovules represents a unique example of trichome development in higher plants. Little is known about the molecular and metabolic mechanisms controlling this process. Here we report a comparative analysis of a fiberless seed (fls) mutant (lacking fibers) and a normal (FLS) mutant to better understand the initial cytological events in fiber development and to analyze the metabolic changes that are associated with the loss of a major sink for sucrose during cellulose biosynthesis in the mutant seeds. On the day of anthesis (0 DAA), the mutant ovular epidermal cells lacked the typical bud-like projections that are seen in FLS ovules and are required for commitment to the fiber development pathway. Cell-specific gene expression analyses at 0 DAA showed that sucrose synthase (SuSy) RNA and protein were undetectable in ils ovules but were in abundant, steady-state levels in initiating fiber cells of the FLS ovules. Tissue-level analyses of developing seeds 15 to 35 DAA revealed an altered temporal pattern of SuSy expression in the mutant relative to the normal genotype. Whether the altered programming of SuSy expression is the cause or the result of the mutation is unknown. The developing seeds of the fls mutant have also shown several correlated changes that represent altered carbon partitioning in seed coats and cotyledons as compared with the FLS genotype.