Preparation of lentinan-calcium carbonate microspheres and their application as vaccine adjuvants
Preparation of lentinan-calcium carbonate microspheres and their application as vaccine adjuvants
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香菇多糖-碳酸钙微球的制备及其作为疫苗佐剂的应用
DOI：
10.1016/j.carbpol.2020.116520
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发表时间：
2020-10-01
影响因子：
11.2
通讯作者：
Wang, Deyun
中科院分区：
文献类型：
--
作者：
Liu, Zhenguang;Yu, Lin;Wang, Deyun
佐剂通过增强免疫原性和维持长期免疫应答来提高疫苗效力。香菇多糖（Lentinan，LNT）是从香菇（Lentinus edodes）中提取的一种具有β-1，6-支链的β-1，3-葡糖六糖，是一种有效的免疫刺激剂。先前的研究已经证明了碳酸钙（CaCO 3）微球的佐剂活性以及它们作为抗原递送系统的用途。在这项研究中，我们成功地加载了碳酸钙微球与LNT和评估其物理化学特性之前，卵清蛋白的吸附。我们的实验结果表明，LNT-CaCO 3显着增强淋巴细胞增殖，并增加脾淋巴细胞中CD 69 + B细胞的频率和CD 4+与CD 8 + T细胞的比例。此外，LNT-CaCO 3出乎意料地诱导免疫小鼠中IgG和Th相关细胞因子（IL-2、IL-4、IFN-γ和TNF-α）的分泌。因此，LNT-CaCO 3微球诱导强大的细胞和体液免疫应答，并具有作为疫苗递送系统的潜在效用。
Adjuvants improve vaccine potency by enhancing immunogenicity and sustaining long-term immune responses. Lentinan (LNT), a beta-1,3-glucohexaose with beta-1,6-branches, is extracted from the mushroom Lentinus edodes and functions as an effective immunostimulatory drug. Previous studies have demonstrated the adjuvant activity of calcium carbonate (CaCO3) microspheres as well as their use as antigen delivery systems. In this study, we successfully loaded CaCO3 microspheres with LNT and evaluated their physicochemical characteristics prior to the adsorption of ovalbumin. Our experimental results demonstrated that LNT-CaCO3 significantly enhanced lymphocyte proliferation, and boosted the frequency of CD69 + B cells and the ratio of CD4+ to CD8 + T cells in spleen lymphocytes. Moreover, LNT-CaCO3 unexpectedly induced the secretion of IgG and Th-associated cytokines (IL-2, IL-4, IFN-gamma, and TNF-alpha) in immunized mice. Therefore, LNT-CaCO3 microspheres induce robust cellular and humoral immune responses and have potential utility as vaccine delivery systems.