Gene expression profiling in an in vitro model of angiogenesis
Gene expression profiling in an in vitro model of angiogenesis
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DOI：
10.1016/s0002-9440(10)65062-6
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发表时间：
2000-06-01
影响因子：
6
通讯作者：
Gerritsen, ME
中科院分区：
文献类型：
--
作者：
Kahn, J;Mehraban, F;Gerritsen, ME
在本研究中，我们使用了一种新颖、全面的 mRNA 分析技术 (GeneCalling) 来确定正在分化为管状结构的人内皮细胞的差异基因表达谱。鉴定出 115 个 cDNA 片段，并显示它们代表 90 个不同的基因。尽管所鉴定的一些基因先前已与血管生成有关，但许多新基因的潜在作用已被揭示，包括 OX-40（白色蛋白同源物）、KIAA0188（血管生成素-2 的同源物）、ADAMTS-4（聚集蛋白聚糖酶-1）和斯钙素。通过废除血管生成抑制剂表达的变化和原位杂交研究证实了对生物学意义的支持。这项研究显着扩展了内皮分化过程中基因表达变化的分子指纹，并为许多新分子在血管生成中的潜在作用提供了新的见解。
In the present study we have used a novel, comprehensive mRNA profiling technique (GeneCalling) for determining differential gene expression profiles of human endothelial cells undergoing differentiation into tubelike structures. One hundred fifteen cDNA fragments were identified and shown to represent 90 distinct genes, Although some of the genes identified have previously been implicated in angiogenesis, potential roles for many new genes, including OX-40, white protein homolog, KIAA0188, a homolog of angiopoietin-2, ADAMTS-4 (aggrecanase-1), and stanniocalcin were revealed. Support for the biological significance was confirmed by the abrogation of the changes in the expression of angiogenesis inhibitors and in situ hybridization studies. This study has significantly extends the molecular fingerprint of the changes in gene expression that occur during endothelial differentiation and provides new insights into the potential role of a number of new molecules in angiogenesis.