MONOCLONAL-ANTIBODIES AGAINST THE MAJOR GLYCOPROTEIN (GP350-220) OF EPSTEIN-BARR VIRUS NEUTRALIZE INFECTIVITY
MONOCLONAL-ANTIBODIES AGAINST THE MAJOR GLYCOPROTEIN (GP350-220) OF EPSTEIN-BARR VIRUS NEUTRALIZE INFECTIVITY
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DOI：
10.1073/pnas.77.9.5307
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发表时间：
1980-01-01
期刊：
影响因子：
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通讯作者：
GEILINGER, K
中科院分区：
文献类型：
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作者：
THORLEYLAWSON, DA;GEILINGER, K
产生产生抗EB病毒（EBV）抗体的杂交瘤[来源于鼠骨髓瘤NS-1和脾细胞]。在EBV生产细胞系上筛选它们产生针对EBV相关膜抗原的抗体。采用~（125）I标记兔抗鼠IG双抗体放射免疫分析法。克隆阳性培养物，并将1个这样的克隆C1用于进一步研究。C1抗体在体外中和EBV，并从膜抗原复合物中免疫沉淀gp 350（B 95 -8 [绒猴肿瘤性白细胞]细胞中的主要糖蛋白）和gp 220（P3 HR-1 [人淋巴瘤]细胞中的主要糖蛋白），表明这些分子具有抗原相关性。用C1抗体通过免疫荧光检测质膜和细胞质中gp 350/220的存在。
Hybridomas [derived from murine myeloma NS-1 and spleen cells] producing antibodies against Epstein-Barr virus (EBV) were produced. They were screened for production of antibodies against the EBV-associated membrane antigens on EBV producer cell lines. A double-antibody radioimmunoassay with 125I-labeled rabbit anti-mouse Ig was used. Positive cultures were cloned and 1 such clone, C1, was used for further study. C1 antibody neutralizes EBV in vitro and immunoprecipitates gp350 (the major glycoprotein in B95-8 [marmoset neoplastic leukocyte] cells) and gp220 (the major glycoprotein in P3HR-1 [human lymphoma] cells) from the membrane antigen complex, suggesting that these molecules are antigenically related. The presence of gp350/220 both on the plasma membrane and in the cytoplasm may be detected by immunofluorescence with C1 antibody.