Distinct patterns of membrane microdomain partitioning in Th1 and Th2 cells
Distinct patterns of membrane microdomain partitioning in Th1 and Th2 cells
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DOI：
10.1016/s1074-7613(01)00223-0
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发表时间：
2001-11-01
期刊：
影响因子：
32.4
通讯作者：
Bottomly, K
中科院分区：
文献类型：
--
作者：
Balamuth, F;Leitenberg, D;Bottomly, K
在这里，我们发现活化的Th1和Th2细胞有不同的膜区隔成脂筏的模式。在Th1细胞而不是Th2细胞中，TOR复合物成员被有效地招募到筏上并在MHC/肽接触部位与筏聚集。在缺乏CD4的情况下，Th1细胞中的TCR/raft关联是缺乏的，这表明CD4有助于TOR向raft募集。我们通过胆固醇消耗研究显示Th1和Th2细胞中筏的不同利用，这改变了Th1而不是Th2细胞中的钙信号传导。此外，Th2细胞对低亲和力肽刺激的反应能力下降。这些研究表明，膜微结构域的成分在功能不同的CD4 T细胞中受到不同的调节。
Here we show that activated Th1 and Th2 cells have distinct patterns of membrane compartmentalization into lipid rafts. TOR complex members are recruited efficiently to rafts and aggregate with rafts at the site of MHC/peptide contact in Th1 cells but not Th2 cells. TCR/raft association in Th1 cells is deficient in the absence of CD4, suggesting that CD4 aids recruitment of the TOR to rafts. We show differential utilization of rafts in Th1 and Th2 cells by cholesterol depletion studies, which alters calcium signaling in Th1 but not Th2 cells. Furthermore, Th2 cells have a decreased ability to respond to low-affinity peptide stimulation. These studies indicate that components of membrane microdomains are differentially regulated in functionally distinct CD4 T cells.