Solubilization of a stereospecific opiate-macromolecular complex from rat brain
Solubilization of a stereospecific opiate-macromolecular complex from rat brain
复制标题
大鼠脑中立体特异性阿片大分子复合物的溶解
作者：
E. J. Simon;J. Hiller;I. Edelman
A [3H]etorphine-macromolecular complex has been solubilized from rat brain synaptosomal fraction by extraction with the nonionic detergent Brij 36T. Stereospecificity of binding to this solubilized complex was demonstrated by the finding that radioactivity in the complex was virtually eliminated when binding had occurred in the presence of excess levorphanol, an active narcotic analgesic, while it was unaffected by its inactive enantiomorph dextrorphan. Bound radioactivity was dissociated by proteolytic enzymes, sulfhydryl reagents, and heat, suggesting the presence of protein. The bound solubilized macromolecular moiety may be the opiate receptor.
