Endothelial cells from diverse tissues exhibit differences in growth and morphology
Endothelial cells from diverse tissues exhibit differences in growth and morphology
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DOI：
10.1006/mvre.1997.2045
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发表时间：
1998-01-01
影响因子：
3.1
通讯作者：
Zink, MC
中科院分区：
文献类型：
--
作者：
Craig, LE;Spelman, JP;Zink, MC
随着人们对内皮细胞在疾病中作用的认识的提高和内皮细胞培养方法的发展，内皮细胞功能的体外研究出现了热潮。然而，最常用于这些研究的细胞不能反映内皮细胞的体内异质性。为了评估来自不同组织的大血管内皮细胞和小血管内皮细胞之间的内在差异，分离绵羊的脑、肺和脂肪组织以及大血管（主动脉），通过乙酰化低密度脂蛋白的荧光激活细胞分选纯化，（DiI-Ac-LDL）标记的细胞，并通过相差和超微结构形态学、血管性血友病因子的表达以及细胞角蛋白、平滑肌肌动蛋白的表达缺乏来表征，和胶质细胞酸性蛋白（GFAP）。虽然所有的内皮细胞培养在相同的培养基中，只有脑微血管内皮细胞显示紧密连接的电子显微镜。只有大血管（主动脉）内皮细胞含有韦伯-帕拉德小体。血管性血友病因子的表达随着细胞传代而降低，但无论培养条件或传代次数如何，DiI-Ac-LDL的摄取始终呈阳性。这些研究表明，微血管和大血管内皮细胞的独特的超微结构特征是固有的细胞本身，而不是由不同的培养条件。该系统允许选择性地影响某些靶器官的内皮细胞的病理过程的研究，并且应该更准确地代表组织特异性内皮细胞在炎症过程中的反应。(C)北京：科学出版社.
An increased recognition of the role of endothelial cells in disease and the development of methods for endothelial cell culture has led to an upsurge in in vitro studies of endothelial cell function. However, the cells most often used for these studies do not reflect the in vivo heterogeneity of endothelial cells. To assess intrinsic differences between large and small vessel endothelial cells from different tissues, primary cultures of endothelial cells from capillaries (brain, lung, and adipose tissue) and a large vessel (aorta) of sheep were isolated, purified by fluorescence-activated cell sorting of acetylated low density lipoprotein (DiI-Ac-LDL) labeled cells, and characterized by phase contrast and ultrastructural morphology, expression of von Willebrand factor, and lack of expression of cytokeratin, smooth muscle actin, and glial fibrillary acidic protein (GFAP). Although all endothelial cells were cultured in the same media, only the brain microvascular endothelial cells demonstrated tight junctions by electron microscopy. Only the large vessel (aortic) endothelial cells contained Weibel-Palade bodies. Expression of von Willebrand factor decreased with passage of cells, but uptake of DiI-Ac-LDL was consistently positive regardless of culture conditions or passage number. These studies demonstrate that the unique ultrastructural characteristics of microvascular and macrovascular endothelial cells are intrinsic to the cells themselves and are not determined by differential culture conditions. This system allows the study of pathologic processes that affect endothelial cells of certain target organs selectively and should more accurately represent the response of tissue-specific endothelial cells in inflammatory processes. (C) 1998 Academic Press.