KANAMYCIN INDUCED OTOTOXICITY IN THE LABORATORY RAT - A COMPARATIVE MORPHOLOGICAL AND AUDIOMETRIC STUDY
KANAMYCIN INDUCED OTOTOXICITY IN THE LABORATORY RAT - A COMPARATIVE MORPHOLOGICAL AND AUDIOMETRIC STUDY
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DOI：
10.1007/bf00310859
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发表时间：
1982-01-01
影响因子：
6.1
通讯作者：
READ, NG
中科院分区：
文献类型：
--
作者：
ASTBURY, PJ;READ, NG
本文报道了卡那霉素[一种抗生素]对Wistar大鼠的行为、听力和组织学影响的两个对比实验。实验一：皮下注射卡那霉素400~1500 mg/kg/d。连续给药20天，对Corti器官的形态损伤进行评估。在第二个实验中，每天给予卡那霉素700 mg/kg，连续4-20天，观察对Corti器官的损害进展。在这两个实验中都进行了阈值漂移的行为测听研究。在第一个研究中，所有动物在最后一次注射后的20天，即第40天后被处死，在第二个研究中，动物组在给药的第4天到第20天之间的4天间隔内被处死。每只动物的一个耳蜗点干，解剖暴露Corti器官，并用扫描电子显微镜进行检查。光镜下观察对侧耳蜗骨的垂直切面。耳蜗学检查结果与行为测听结果进行对照。Corti器官的形态损伤遵循老套的变性模式，其程度似乎由卡那霉素给药的次数和浓度决定。附带的听力检查表明，在通过观察Preyer反射可以检测到行为听阈的变化之前，已经发生了广泛的损害。
Two comparative experiments on the behavioral, audiometric and histological effects of kanamycin[an antibiotic]-induced cochleotoxicity in the Wistar rat are reported. In the 1st experiment kanamycin 400-1500 mg/kg per day was injected s.c. for 20 days and the morphological damage to the organ of Corti assessed. In the 2nd experiment the progression of damage to the organ of Corti was examined in animals given kanamycin 700 mg/kg per day for 4-20 days. Behavioral audiometric studies of threshold shift were undertaken throughout both experiments. In the 1st study, all the animals were killed after a recovery period of 20 days from the last injection, i.e., day 40, and in the 2nd study groups of animals were killed at 4-day intervals between days 4 and 20 of dosing. One cochlea from each animal was critical point-dried, dissected to expose the organ of Corti and examined by scanning electron microscopy. A vertical section through the contralateral cochlea was examined by light microscopy. The results of the morphological examination of the cochleas were collated with the behavioral audiometry. The morphological damage to the organ of Corti followed a stereotyped pattern of degeneration, the extent of which appeared to be determined both by the number and concentration of the kanamycin administrations. The collateral audiometric examinations indicated that extensive damage had taken place before a shift in the behavioral auditory threshold could be detected by observation of the Preyer reflex.