In Vitro Analyses of Endothelial Cell Permeability
In Vitro Analyses of Endothelial Cell Permeability
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DOI：
10.1007/978-1-61779-191-8_19
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发表时间：
2011-01-01
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影响因子：
--
通讯作者：
Wittchen, Erika S.
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文献类型：
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作者：
Monaghan-Benson, Elizabeth;Wittchen, Erika S.
内衬脉管系统的血管的内皮细胞和连接它们的细胞细胞连接部为血流和组织之间的流体、免疫细胞和大分子的通过提供主要屏障。在正常的生理过程中，需要对这种屏障进行适当和动态的调节;然而，如果不严格控制，内皮的通透性增加也会导致许多病理情况，包括慢性炎症性疾病和水肿。开发体外方法来研究内皮屏障功能已成为确定许多这些疾病状态的分子机制的关键。在这一章中，我们描述了三种互补的方法来测量内皮单层通透性和屏障功能在体外。
Endothelial cells lining the vessels of the vasculature and the cell cell junctions, which join them, provide the primary barrier to the passage of fluids, immune cells, and macromolecules between the bloodstream and the tissues. Appropriate and dynamic regulation of this barrier is required during normal physiological processes; however, if not tightly controlled, increased permeability of the endothelium can also contribute to many pathological situations, including chronic inflammatory diseases and edema. The development of in vitro methods to study endothelial barrier function has been key in the identification of molecular mechanisms underlying many of these disease states. In this chapter, we describe three complementary approaches to measure endothelial monolayer permeability and barrier function in vitro.