IONIC PROBES OF MEMBRANE STRUCTURES
IONIC PROBES OF MEMBRANE STRUCTURES
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DOI：
10.1111/j.1749-6632.1972.tb54807.x
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发表时间：
1972-01-01
影响因子：
5.2
通讯作者：
EISENMAN, G
中科院分区：
文献类型：
--
作者：
SZABO, G;MCLAUGHLIN, SG;EISENMAN, G
在本文中，我们研究了识别那些调节膜离子渗透性的膜结构变量（极性头基团和碳氢化合物尾部的性质）的可能性。改变双层脂质组成会产生物理参数（表面电位、分配系数和迁移率）的变化，这些物理参数控制着阴离子和阳离子中性载体介导的电导。具体地说，通过形成弥漫性双层而产生的表面电势表明，带电极性头基团的影响是可以理解的，而粘度的影响可以通过“冻结”膜来证明。膜成分对膜电导率的影响通过第三个不太为人所知的例子来说明，即胆固醇如何改变双层电导率。结果表明，利用正、负渗透种作为膜结构探针是可行的。
In this paper we have examined the possibility of identifying those membrane structural variables (polar head groups and the nature of hydrocarbon tails) that modulate membrane ionic permeability. Altering the bilayer lipid composition produces variations in physical parameters (surface potential, partition coefficient, and mobility) governing the conductance mediated by neutral carriers of anions and cations. Specifically, the effects of the charged polar head groups are shown to be understandable in terms of the surface potential they produce through the formation of a diffuse double layer, whereas the effects of the viscosity may be demonstrated by “freezing” the membrane. The effects of membrane composition on membrane conductance are illustrated by a third, less well understood, example of how cholesterol alters bilayer conductances. The results indicate the possibility of using positive and negative permeant species as probes of membrane structures.