Cell-to-cell signaling in the symbiotic nitrogen-fixing bacterium Rhizobium leguminosarum: Autoinduction of a stationary phase and rhizosphere-expressed genes
Cell-to-cell signaling in the symbiotic nitrogen-fixing bacterium Rhizobium leguminosarum: Autoinduction of a stationary phase and rhizosphere-expressed genes
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DOI：
10.1128/jb.178.2.372-376.1996
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发表时间：
1996-01-01
影响因子：
3.2
通讯作者：
Greenberg, EP
中科院分区：
文献类型：
--
作者：
Gray, KM;Pearson, JP;Greenberg, EP
Sym质粒pRL1JI编码豆根瘤菌形成固氮根瘤的功能。其中一些结瘤基因参与识别植物根产生的化学信号，另一些基因则是产生植物识别的化学信号所必需的。pRL1JI还包含一个调控基因rhiR，它与鱼类弧菌发光基因的转录激活因子LuxR同源。LuxR的活性需要一种信号化合物，一种自动诱导剂。我们已经鉴定出一种豆科拟青霉自身诱导子，它与RhiR一起激活根际表达的rhiABC操纵子和由pRL1JI编码的生长抑制功能，这种胞间信号是一种N-酰化的高丝氨酸内酯，在结构上与费斯切里乳杆菌和其他自体诱导剂有关。这些发现表明根瘤形成中的细胞间通讯达到了一个新的水平。
The Sym plasmid pRL1JI encodes functions for the formation of nitrogen-fixing pea root nodules by Rhizobium leguminosarum. Some of the nodulation genes are involved in recognition of chemical signals produced by the plant root, and others are required for production of chemical signals recognized by the plant, pRL1JI also contains a regulatory gene, rhiR, that is homologous to luxR, the transcriptional activator of luminescence genes in Vibrio fischeri. LuxR requires a signal compound, an autoinducer, for its activity. We hale identified an R. leguminosarum autoinducer that, together with RhiR, is required to activate both the rhizosphere-expressed rhiABC operon and a growth-inhibiting function encoded by pRL1JI, This intercellular signal is an N-acylated homoserine lactone structurally related to the V. fischeri and other autoinducers. These findings indicate a new level of intercellular communication in root nodule formation.